Physicochemical characteristics and toxicity of nickel oxide particles calcined at different temperatures

  • Sentaro Takahashi
  • Masahiro Oishi
  • Eriko Takeda
  • Yoshihisa Kubota
  • Tadashi Kikuchi
  • Keiichi Furuya
Original Articles


The physicochemical characteristics and cytotoxicity of two types of commercial nickel oxide particles (black and green nickel oxide) and five types of nickel oxide particles prepared by calcination of the black nickel oxide at 600–1000°C were studied. Thermal analysis with mass spectroscopy showed that the black nickel oxide particles contained approximately 1.4% impurity, which seemed to be basic nickel carbonate. The calcination treatment at 600°C increased the nickel content and decreased the oxygen content, but these remained constant in the particles treated at higher temperatures (700–1000°C) and in the green nickel oxide particles. The water solubility of black nickel oxide particles was markedly greater than that of the other particles, especially in the first 24 h after mixing with water. The solubility of the calcined particles decreased with increasing calcination temperature. The cytotoxicity of these particles was evaluated by the viability of rat alveolar macrophages and by the inhibition of cell proliferation in Chinese hamster ovary cells. The black nickel oxide was the most cytotoxic of the particles examined, and this may be attributable, at least in part, to a rapid dissolution of nickel from the contained impurity. The toxicity of the calcined particles decreased with increasing calcination temperature. These results indicate that water solubility, which depends on calcination temperature, modulates the acute cytotoxicity of nickel oxide particles.

Index Entries

Nickel oxide physicochemical characteristic calcination temperature cytotoxicity 


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Copyright information

© Humana Press Inc 1999

Authors and Affiliations

  • Sentaro Takahashi
    • 1
  • Masahiro Oishi
    • 2
  • Eriko Takeda
    • 2
  • Yoshihisa Kubota
    • 1
  • Tadashi Kikuchi
    • 2
  • Keiichi Furuya
    • 2
  1. 1.The 4th Research GroupNational Institute of Radiological SciencesChibaJapan
  2. 2.Department of Applied Chemistry, Faculty of ScienceScience University of TokyoTokyoJapan

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