Skip to main content
SpringerLink
Log in

Tailor-made agarose-based reactive beads for hemoperfusion and plasma perfusion

  • Adsorbents and Immunosorbents
  • Published:
Applied Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

Composite beads of approximately 1mm diameter, made of cross-linked agarose and containing Fuller’s Earth or zirconium oxide powders, were prepared and used in extracorporeal systems for blood detoxification. The former was used for the removal of Paraquat, while the latter was used to remove inorganic phosphate from hyper-phosphatamic animals with or without acute renal failure. The high surface area of the powder, combined with the low resistance to diffusion in the cross-linked agarose matrix, are highly advantageous. The crosslinking provides high mechanical strength, heat stability, prolonged shelf life, good blood flow characteristics, and prevents the release of fine particles into the blood.

Crosslinked agarose beads of 1 mm diameter, containing chemically-bound heparin were also prepared, and used as a model for direct contact removal of LDL-cholesterol from the blood of familial hypercholesterolemic patients by hemoperfusion. The high capacity of these beads (over 5 mg LDL/mL beads) indicates that this clinical modality can replace the highly expensive plasmapheresis procedure presently used.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Yatzidis, H. (1964),Proc. Eur. Dial. Transpl. Assoc 1, 83.

    Google Scholar 

  2. Chang, T. M. S. (1964),Science 146, 524.

    Article  CAS  Google Scholar 

  3. Chang, T. M. S. (1977), inArtificial Organs, Kenedi, R., Courtney, J., Gaylor, J. S., and Gilchrist, T., eds., Macmillan, London, pp. 164–177.

    Google Scholar 

  4. Fennimore, J., Kolthamer, J. C., and Lang, S. M. (1977), inArtificial Organs, Kenedi, R., Courtney, J., Gaylor, J. S., and Gilchrist, T., eds. Macmillan, London, pp. 148–157.

    Google Scholar 

  5. Okonek, S. (1980), inHemoperfusion: Kidney and Liver Support and Detoxification, Sideman, S., and Chang, T. M. S., eds., Hemisphere-McGraw-Hill Co., NY, pp. 219–228.

    Google Scholar 

  6. Sideman, S., Mor, L., Rousseau, I., Brandes, J. M., and Ben-Arie, D. (1977), inArtificial Organs, Kenedi, R., Courtney, J., Gaylor, J. S., and Gilchrist, T., eds., Macmillan, London, pp. 413–424.

    Google Scholar 

  7. Cooney, D. D., Infantolino, W., and Kane, R. (1979),Biomat. Med. Dev. Art. Org. 7, 443.

    CAS  Google Scholar 

  8. Voland, H. W., Schirop, T., Barkow, D., and Ibe, K. (1980), inHemoperfusion: Kidney and Liver Support and Detoxification, Sideman, S., and Chang, T. M. S., eds., Hemisphere-McGraw-Hill Co., NY, pp. 319–326.

    Google Scholar 

  9. Malchesky, P. S., Varne, W., Piatkiewicz, S. and Nose, Y. (1977),Trans. Am. Soc. Artif. Intern. Organs 23, 659.

    CAS  Google Scholar 

  10. Malchesky, P. S., Asanuma A., Smith, J., Zawicki, I., and Nose, Y. (1980), in:Hemoperfusion: Kidney and Liver Support and Detoxification, Sideman, S., and Chang, T. M. S., eds., Hemisphere-McGraw-Hill Co., NY, pp. 111–119.

    Google Scholar 

  11. Randerson, D. H., Gurland, H. J., Schmidt, B., Farrell, P. C., Hone, P. W. E., Stokoe, C., Zuber, A., Blogg, A., Fatheh-Moghadam, A., Marschner, I., and Kopcke, W. (1982), inHemoperfusion, Bonomini, V., and Chang, T. M. S., eds., Karger, Basel.Contr. Nephrol. 29, 53

    Google Scholar 

  12. Tangerman, A., Snel, P., Verkooyen, Ad. H. M., Hectors, M. P. C., and von Tongeren, J. H. M. (1982),Artif. Organs 4, 302.

    Article  Google Scholar 

  13. van Berlo, A., Verkooyen, A., and Tangerman, A. (1982),Int. J. Artif. Organs,5, 181.

    Google Scholar 

  14. Chang, T. M. S. (1976), inMethods in Enzymology vol, 44, Mosbach, K., ed., Academic Press, New York, p. 676.

    Google Scholar 

  15. Chang, T. M. S. (1977), inBiomedical Application of Immobilized Enzymes and Proteins, Chang, T. M. S., ed., vol. I, Plenum Press, New York, p. 147.

    Google Scholar 

  16. Shu, C. D., and Chang, T. M. S. (1980),Int. J. Artif. Organs 3, 287.

    CAS  Google Scholar 

  17. Grunwald, J., and Chang, T. M. S. (1980), inHemoperfusion: Kidney and Liver Support and Detoxification, Sideman, S., and Chang, T. M. S., eds., Hemisphere-McGraw-Hill Co., NY pp. 389–394.

    Google Scholar 

  18. Chang, T. M. S. (1980),Trans. Am. Soc. Artif. Int. Organs 26, 546.

    CAS  Google Scholar 

  19. Arnott, S., Fulmer, A., Scott, W. E., Dea, I. C. M., Moorhouse, R., and Rees, D. A. (1974),J. Mol. Biol. 90, 269.

    Article  CAS  Google Scholar 

  20. Porath, J., Jonson, J. C., and Laas, I. (1971),J. Chromatogr. 60, 167.

    Article  CAS  Google Scholar 

  21. Tabak, A., Lotan, N., Sideman, S., and Taitelman, U. (1983),Artif. Organs,7, 146.

    Article  Google Scholar 

  22. Sideman, S., Tatelman, U., Tabak, A., Lotan, N., Mihich, M., and Lupovich, S. (1982),Vet. Hum. Tox.,24–5, 43.

    Google Scholar 

  23. Laas, T. (1975),J. Chromatogr. 111, 373.

    Article  CAS  Google Scholar 

  24. deKoning, H. W. M., Chamuleau, R. A. F. M., and Bantjes, A. (1982),Artif. Organs 6, 280.

    Article  CAS  Google Scholar 

  25. Hughes, R. D., Dunlop, E. H., Davis, M., and Silk, D. B. A. (1977),Biomat. Med. Dev. Artif. Organs 5, 205.

    CAS  Google Scholar 

  26. Lupien, P. J., Moorjani, S., and Awad, J. (1976),Lancet ii, 1261.

    Article  Google Scholar 

  27. Moorjani, S., Lupien, P. J., and Awad, J. (1977),Clin. Chim. Acta 77, 21.

    Article  CAS  Google Scholar 

  28. Brunner, G., and Loesgen, H. (1977), inArtificial Organs, Kenedi, R., Courtney, J., Gaylor, J. S., and Gilchrist, T., eds., Macmillan, London, pp. 388–394.

    Google Scholar 

  29. Brunner, G., Harstick, K., and Holloway, C. J. (1980), inHemoperfusion: Kidney and Liver Support and Detoxification, Sideman, S. and Chang, T. M. S., eds., Hemisphere-McGraw-Hill Co., NY, pp. 37–44.

    Google Scholar 

  30. Xu, C. X., Tang, X. J., Niu, Z., and Li, Z. N. (1981),Int. J. Artif. Organs 4, 200.

    CAS  Google Scholar 

  31. Jonsson, S., and Hakansson, L. (1981),Artif. Organs 5 (Abstr.), 34.

    Google Scholar 

  32. Jonsson, S., Hakansson, L., and Nylen, U. (1981),Artif. Organs 5 (Abstr.), 34.

    Google Scholar 

  33. Burgstaler, E. A., Pineda, A. A., and Ellefson, R. D. (1980),Mayo Clin. Proc. 55, 180.

    CAS  Google Scholar 

  34. Schmer, G., Rastelli, L., Dennis, M. B., Hershon, K., Young, J., and Chait, A. (1981),Trans. Am. Soc. Artif. Organs 27, 547.

    CAS  Google Scholar 

  35. Sideman, S., Lotan, N., Tabak, A., Mor, L., Duveborg, L., Bleiberg, B., and Brook, J., to be published (1983).

  36. Stoffel, W., and Demant, T. (1981),Proc. Natl. Acad. Sci. US 78, 611.

    Article  CAS  Google Scholar 

  37. Stoffel, W., Borberg, W., and Greve, V. (1981), Lancetii, 1005.

    Article  Google Scholar 

  38. Sideman, S., Hoffer, E., and Brunner, G. (1980), Technion R & D Found. Ltd., Proj. 071-182, Rept. No. 72 to MEP Found, Women’s Division, ATS, NY, USA.

  39. Sideman, S., Manor, D., Hoffer, E., Tabak, A., Lotan, N., Mordochovich, D. and Brunner, G. (1983),Life Support Syst. 1, 113.

    CAS  Google Scholar 

  40. Nilsson, K., and Mosbach, K. (1980),Eur. J. Biochem. 112, 397.

    Article  CAS  Google Scholar 

  41. Clerk, D. G. (1971),Brit. J. Ind. Med. 28, 186.

    Google Scholar 

  42. Amphlett, C. B. (1964),Inorganic Ion Exchangers, Elsevier, Amsterdam, pp. 42–56.

    Google Scholar 

  43. Sideman, S., Hoffer, E., Better, O. S., and Lupovich, S. (1980), inHemoperfusion: Kidney and Liver Support and Detoxification, Sideman, S., and Chang, T. M. S., eds., Hemisphere-McGraw-Hill Co., NY, pp. 81–90.

    Google Scholar 

  44. Denti, E., Walker, J. M., Brancaccio, D., and Tessore, V. (1977),Med. Instr. 11, 212.

    CAS  Google Scholar 

  45. Gurland, H. J., Castro, L. A., Samtleben, W., and Fernandez, J. C. (1978),Proc. Conf. Nondialytic Management Uremia, Brooklyn, NY.

  46. Sideman, S., and Manor, D. (1982),Nephron 31, 1.

    CAS  Google Scholar 

  47. Alfrey, A. C., Le Gendre, G. R., and Kaehny, W. D. (1976),N. Engl. J. Med. 294, 184.

    Article  CAS  Google Scholar 

  48. Lewin, A. J., Greenbaum, M. A., Gordon, A., and Maxwell, M. H. (1972),Proc. Dialysis Transplant. Forum, p. 52.

  49. Harrison, J. W. E., Trabin, B., and Martin, E. W. (1951),J. Pharmacol. Exp. Therapy 102, 179.

    Google Scholar 

  50. Clinton, L. T., and Shubert, J. (1948),J. Pharmacol. Exp. Therapy 94, 1.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Departments of Chemical and Biomedical Engineering and Faculty of Medicine, Isreal Institute of Technology, 32000, Haifa, Israel

    S. Sideman, N. Lotan, A. Tabak, D. Manor, L. Mor, U. Taitelman, J. Brook & A. Tzipiniuk

  2. Julius Silver Institute of Biomedical Engineering, Technion, Israel Institute of Technology, 32000, Haifa, Israel

    S. Sideman, N. Lotan, A. Tabak, D. Manor, L. Mor, U. Taitelman, J. Brook & A. Tzipiniuk

Authors
  1. S. Sideman
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. N. Lotan
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. Tabak
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. D. Manor
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. L. Mor
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. U. Taitelman
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. J. Brook
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. A. Tzipiniuk
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Sideman, S., Lotan, N., Tabak, A. et al. Tailor-made agarose-based reactive beads for hemoperfusion and plasma perfusion. Appl Biochem Biotechnol 10, 167–182 (1984). https://doi.org/10.1007/BF02783749

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02783749

Index Entries

Search

Navigation