Abstract
Estimation of the optimal concentrations of residual sugar in medium for a fed-batch culture of Baker’s yeast has been studied and practiced. The concentrations, however, depended on different species and targets of the biomass, which was expected to be made. Kinetic changes of the residual phosphate salt in the medium conformed to a logarithmic process until the fourth hour during an 11-h culture. The parabolic method (see ref. 9 later in article) might be qualified to maintain the concentrations of residual sugar around 0.15 g/L. It was demonstrated that cell growth followed a sigmoid process during a fed-batch culture, because the cells consumed the nutrient with two metabolic pathways, one was for cell conversion and another was for non-cell conversion. With the parabolic method, we can estimate kinetics of cell growth and cell growth rate during the culture.
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Abbreviations
- ADY:
-
active dry yeast
- GPR:
-
gas production rate
- CY:
-
compressed yeast
- RQ:
-
respiratory quotient
- α:
-
cell conversion quotient (liter2): biomass produced from consumption of 1 g sugar
- β:
-
non-cell conversion quotient (liter/g): byproducts produced from consumption of 1 g sugar
- μ:
-
specific growth rate (h-1)
- Ψ:
-
consumption parameter (g2)
- x:
-
concentration of residual sugar in medium (g liter-1)
- Vt :
-
total nutrient consumption rate (v/h)
- Vi :
-
nutrient consumption rate on reproducible growth (v/h)
- VJ :
-
nutrient consumption rate by Pasteur effect, Crabtree effect, and production rate of byproducts (v/h)
- Vc :
-
cell growth rate (g/h)
- Mc :
-
biomass (g)
- t :
-
time (h)
References
Crabtree, H. G. (1929),Biochem. J. 23, 536–545.
Tayeb, A. M., Ashour, I. A., and Mostafa, N. A. (1991),Energy Convers. Mgmt. 32, 491–497.
Chen, S. Y. and Xiao, X. P. (1990), inBiochemistry of the Yeast, 1st Ed., Du Xiu-Ming and Shi Hong-Ying, eds., Academic Press of Shan Dong, Ji-Nan City, pp. 155–178.
Fredrickson, A. G., Megee, R. D., and Tsuchiya, H. M. (1970), inAdvances in Applied Microbiology, vol. 13, Perlman, D., ed., Academic, New York, pp. 419–464.
Monod, J. (1949),Ann. Rev. Microbiol. 3, 371–1949.
Fang, B. S. and Lin, J. Q. (1992),Chin. J. Biotechnol. 8, 283–287.
Mignone, C. F. and Rossa, A. C. (1993),Proc. Biochem. 28, 405–410.
Xu, H., Kang, Y. X., Jiang, H. X., Chen, X. D., Wang, L., and Zhou, J. (1989),Sci. Sin. 32, 822–829.
He Rong-Qiao, Xu Jung, Li Chuan-You and Zhao Xiu-An (1993),Appl. Biochem. Biotech. 41, 145–155.
Oura, E., Suomalanen, H., and Viskari, R. B. (1982),Fermented Food, vol.7, Rose, A. H., ed., Academic, London, pp. 87–146.
Reed, G. and Peppier, H. J. (1973),Yeast Technology, AVI, Westport, CT, pp. 103–164.
Reed, G. (1982), inPrescott and Dunn’s Industrial Microbiology, AVI, Westport, CT, pp. 593–634.
Reiff, F. (1962),Die Hefen Bans II. Technologie Der Hefen. Verlag Hans Carl, Nurnberg, Backhe-fefabrikation, pp. 501–610.
White, J. (1954), inYeast Technology, Chapman and Hall, 1st ed., Jarrold, London, pp. 13–27.
Mazia, D. (1961), inThe Cell Biochemistry, Physiology, Morphology, Brachet, J. and Mirsky, A. E., eds., Academic, New York, pp. 98–111.
Park, S. C. and Baratti, J. (1991),Appl. Microbiol. Biotechnol. 35, 283–291.
He, R. Q., Zhang, G. J., and Yao, J. L. (1995),Biochem. Mol. Biol. Int. 36, 1–12.
Xu, H. and Kang, Y. X. (1985),Microbiology Acta Sinica 12, 187–190.
ld Health Organization (WHO)(1980), inManual of Basic Techniques for a Health Laboratory, Geneva, pp. 360–366.
Felsher, A. R., Koch, R. B., and Lorsen, R. A. (1955),Cereal Chem. 32, 117–124.
Ballentine, R. (1957), inMethods in Enzymology, vol. 3, Colowick, S. and Kaplan, N. O., eds., Academic, New York, pp. 984.
Fiske, C. H. and Subbarow, Y. P. (1925),J. Biol. Chem. 66, 375–381.
Wang, D. I. C., Cooney, C. L., Demain, A. L., Dunnill, P., Humphrey, A. E., and Lilly, M. D. (1979), inFermentation and Enzyme Technology, John Wiley, New York, pp. 57–97.
Suomalainen, H. and Oura, E. (1971), inThe Yeast, vol. II, Ross, A. H. and Harrison, J. S., eds., Academic, London, 271–309.
He Rong-Qiao (1995),Exploration of Nature 14, 40–46.
Alberta, B., Bray, D., Lewis, J., Raff, M., Roberts, K., and Watson, J. D. (1983), inMolecular Biology of the Cell, Garland, New York, pp. 611–672.
Ambrose, J. J. and Easty, D. M. (1971), inCell Biology, Thomas Nelson, London, pp. 289–290.
Edwards, C. (1981), The microbial cell cycle.Lecture in Microbiology at the University of Liverpool, Hong Kong, 1st ed., pp. 4–10.
Nord, F. F. and Weiss, S. (1958), inThe Chemistry and Biology of Yeast, Cool, A. H., ed., Academic, New York, pp. 323–362.
Morris, E. O. (1958), inThe Chemistry and Biology of Yeast, Cook, A. H., ed., Academic, New York, pp. 251–317.
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He, RQ., Li, CY., Xu, J. et al. Estimation of the optimal concentrations of residual sugar and cell growth rate for a fed-batch culture ofSaccharomyces cerevisiae . Appl Biochem Biotechnol 60, 229–244 (1996). https://doi.org/10.1007/BF02783586
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DOI: https://doi.org/10.1007/BF02783586