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Enzymatic hydrolysis of starch in water-immiscible organic solvent, two-phase systems

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Abstract

Enzyme-catalyzed hydrolyzations of starch by α-amylase have been studied in various two-phase systems, consisting of water and a water-immiscible organic solvent. The hydrolytic conversion of soluble starch to malto-oligosaccharides by α-amylase was greatly accelerated in 10% (v/v) water content of water-dodecane two-phase systems. However, a rapid inactivation of the enzyme has been observed in these systems. Addition of surfactant to these systems, such as polyoxyethylene (20) sorbitan monopalmitate (Tween 60) or bis(2-ethylhexyl) sodium sulfosuccinate (AOT), was effective for the enzyme stability. Effects of enzyme immobilization on the stability of α-amylase, using Ca-alginate and chitosan beads, also have been studied. The stability of immobilized enzyme was clearly enhanced in a 5–10% (v/v) water content two-phase system, whereas the free enzyme was inactivated within 41 h, remaining at a relative activity of 47–76% after 41 h of treatment. Furthermore, scanning electron micrographs (SEM) were taken to observe the effect of the two-phase system on the hydrolysis of starch. Potato starch granules have been extremely swelled and burst out in the stirred 10% (v/v) water content system, which did not contain enzymes.

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Authors and Affiliations

  1. Akebono Brake Research & Development Center Ltd., Higashi Hanyu, Saitama Pref., Japan

    Takashi Morita

  2. Research Center for Advanced Science and Technology, University of Tokyo, 4-6-1 Komaba, Meguro-ku, 153, Tokyo, Japan

    Takashi Morita & Isao Karube

Authors
  1. Takashi Morita
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  2. Isao Karube
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Morita, T., Karube, I. Enzymatic hydrolysis of starch in water-immiscible organic solvent, two-phase systems. Appl Biochem Biotechnol 55, 75–86 (1995). https://doi.org/10.1007/BF02783550

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  • DOI: https://doi.org/10.1007/BF02783550

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