One-step electroblotting of 2- to 100-kDa proteins onto a PVDF membrane
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This article describes a method for electroblotting peptides and small proteins (< 100 kDa) from tricine gels onto a PVDF membrane. The major potential problem with these types of procedures is that proteins tend to stay in the gel under conditions where peptides are effectively eluted. The suggested protocol allows the complete transfer and binding of proteins and peptides in the range of 2–97 kDa.
Index EntriesElectroblotting proteins peptides PVDF
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