Applied Biochemistry and Biotechnology

, Volume 53, Issue 3, pp 285–292 | Cite as

One-step electroblotting of 2- to 100-kDa proteins onto a PVDF membrane

  • Juan Parrado
  • Antonio Ayala
  • Alberto Machado
Original Articles


This article describes a method for electroblotting peptides and small proteins (< 100 kDa) from tricine gels onto a PVDF membrane. The major potential problem with these types of procedures is that proteins tend to stay in the gel under conditions where peptides are effectively eluted. The suggested protocol allows the complete transfer and binding of proteins and peptides in the range of 2–97 kDa.

Index Entries

Electroblotting proteins peptides PVDF 


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Copyright information

© Humana Press Inc 1995

Authors and Affiliations

  • Juan Parrado
    • 1
  • Antonio Ayala
    • 1
  • Alberto Machado
    • 1
  1. 1.Departamento de Bioquimica, Bromatologia y Toxicologia, Facultad de FarmaciaUniversidad de SevillaSevillaSpain

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