Advertisement

Applied Biochemistry and Biotechnology

, Volume 53, Issue 3, pp 215–227 | Cite as

A colorimetric fructose assay

  • Kimberley A. C. C. Taylor
Original Articles

Abstract

A new spectrophotometric method for measuring fructose is presented. The method uses Tryptamine in HCl acid, is carried out at 60°C, and is complete within 60 min. The assay is read at 518 nm and shows very low interference from other sugars. The method can be used for fructose, fructosans, and inulin.

Index Entries

Fructose fructosan inulin tryptamine colorimetric assay sugar analysis 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    McDonald, P. (1981),The Biochemistry of Silage, John Wiley, New York.Google Scholar
  2. 2.
    Pogell, B. M. (1954),J. Biol. Chem. 211, 143–147.Google Scholar
  3. 3.
    Messineo, L. and Mussara, E. (1972),Int. J. Biochem. 3, 691–699.CrossRefGoogle Scholar
  4. 4.
    Dubois, M., Gilles, K. A., Hamilton, J. K., Rebers, P. A., and Smith, F. (1956),Anal. Chem. 28, 350–356.CrossRefGoogle Scholar
  5. 5.
    Ashwell, G. (1957), inMethods in Enzymology, vol.3, Colwick, S. P. and Kaplan, N. O., eds., Academic, New York, pp. 73–105.Google Scholar
  6. 6.
    de Carvalho, C. A. and Pogell, B. M. (1957),Biochem. Biophys. Acta 26, 206.CrossRefGoogle Scholar

Copyright information

© Humana Press Inc 1995

Authors and Affiliations

  • Kimberley A. C. C. Taylor
    • 1
  1. 1.Department of Animal and Poultry ScienceUniversity of GuelphGuelphCanada

Personalised recommendations