A modification of the phenol/sulfuric acid assay for total carbohydrates giving more comparable absorbances
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The conditions and acid strength of the phenol/sulfuric acid assay were investigated to improve agreement between absorbances obtained from different sugars. It was found that by increasing acid strength and by cooling the tubes in water after a short reaction time, the values obtained for several sugars, including fructose and xylose, agreed, on an equimolar value, with that for glucose.
Index EntriesSugar analysis carbohydrate colorimetric assay phenol-sulfuric acid
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