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Applied Biochemistry and Biotechnology

, Volume 53, Issue 3, pp 207–214 | Cite as

A modification of the phenol/sulfuric acid assay for total carbohydrates giving more comparable absorbances

  • Kimberley A. C. C. Taylor
Original Articles

Abstract

The conditions and acid strength of the phenol/sulfuric acid assay were investigated to improve agreement between absorbances obtained from different sugars. It was found that by increasing acid strength and by cooling the tubes in water after a short reaction time, the values obtained for several sugars, including fructose and xylose, agreed, on an equimolar value, with that for glucose.

Index Entries

Sugar analysis carbohydrate colorimetric assay phenol-sulfuric acid 

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References

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    Du Bois, M., Gilles, K. A., Hamilton, J. K., Rebers, P. A., and Smith, F. (1956),Anal. Chem. 28, 350–356.CrossRefGoogle Scholar
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    Rao, P. and Pattabiraman, T. N. (1989),Anal. Biochem. 181, 18–22.CrossRefGoogle Scholar
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    Rao, P. and Pattabiraman, T. N. (1990),Anal. Biochem. 189, 178–181.CrossRefGoogle Scholar

Copyright information

© Humana Press Inc 1995

Authors and Affiliations

  • Kimberley A. C. C. Taylor
    • 1
  1. 1.Department of Animal and Poultry ScienceUniversity of GuelphGuelphCanada

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