Comparison of reactivity of alkane hydroxylation with intact cells and cell- free extracts ofMethylosinus trichosporium OB3b
Kinetic studies for hydroxylation of a series of alkanes (methane, ethane and propane) with intact cells and cell-free extracts ofMethylosinus trichosporium OB3b were carried out.K m values for alkane hydroxylation with cell-free extracts were lower than those with intact cells, suggesting that cytoplasm plays an important role in the solubility of alkanes to increase their concentration.
Index EntriesAlkane hydroxylation methane monooxygenase Methylosinus trichosporium OB3b
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