Applied Biochemistry and Biotechnology

, Volume 53, Issue 3, pp 199–205 | Cite as

Comparison of reactivity of alkane hydroxylation with intact cells and cell- free extracts ofMethylosinus trichosporium OB3b

  • Daisuke Sugimori
  • Ryota Ando
  • Ichiro Okura
Original Articles


Kinetic studies for hydroxylation of a series of alkanes (methane, ethane and propane) with intact cells and cell-free extracts ofMethylosinus trichosporium OB3b were carried out.K m values for alkane hydroxylation with cell-free extracts were lower than those with intact cells, suggesting that cytoplasm plays an important role in the solubility of alkanes to increase their concentration.

Index Entries

Alkane hydroxylation methane monooxygenase Methylosinus trichosporium OB3b 


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  1. 1.
    Anthony, C. (1982), inThe Biochemistry of Methylotrophs, Academic, London, pp. 152–194.Google Scholar
  2. 2.
    Higgins, I. J., Best, D. J., and Hammond, R. C. (1980),Nature 286, 561–564.CrossRefGoogle Scholar
  3. 3.
    Shimoda, M. and Okura, I. (1991),J. Mol. Catal. 64, L23-L25.CrossRefGoogle Scholar
  4. 4.
    Johannes Frank, J., van Krimpen, S. H., Verwiel, P. E. J., Jongejan, J. A., Mulder, A. C., and Duine, J. A. (1989),Eur. J. Biochem. 184, 187–195.CrossRefGoogle Scholar
  5. 5.
    Fox, B. G., Froland, W. A., Jollie, D. R., and Lipscomb, J. D. (1990),Methods Enzymol. 188, 191–202.CrossRefGoogle Scholar
  6. 6.
    Fox, B. G., Froland, W. A., Dege, J. E., and Lipscomb, J. D. (1989),J. Biol. Chem. 264, 10,023-10,033.Google Scholar
  7. 7.
    Sugimori, D. and Okura, I. (1994),J. Mol. Catal. 93, 119–124.CrossRefGoogle Scholar

Copyright information

© Humana Press Inc 1995

Authors and Affiliations

  • Daisuke Sugimori
    • 1
  • Ryota Ando
    • 1
  • Ichiro Okura
    • 1
  1. 1.Department of BioengineeringTokyo Institute of TechnologyYokohamaJapan

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