Abstract
Kinetic studies for hydroxylation of a series of alkanes (methane, ethane and propane) with intact cells and cell-free extracts ofMethylosinus trichosporium OB3b were carried out.K m values for alkane hydroxylation with cell-free extracts were lower than those with intact cells, suggesting that cytoplasm plays an important role in the solubility of alkanes to increase their concentration.
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Sugimori, D., Ando, R. & Okura, I. Comparison of reactivity of alkane hydroxylation with intact cells and cell- free extracts ofMethylosinus trichosporium OB3b. Appl Biochem Biotechnol 53, 199–205 (1995). https://doi.org/10.1007/BF02783495
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DOI: https://doi.org/10.1007/BF02783495