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Applied Biochemistry and Biotechnology

, Volume 53, Issue 3, pp 195–197 | Cite as

A strategy to sequence repetitive DNA based on partial restriction enzyme cleavage

  • F. G. C. Abath
  • A. A. Holder
Accelerated Article

Abstract

The strategy to sequence repetitive DNA described in this article is based on partial restriction enzyme cleavage. It is an alternative to using nested deletion with exonuclease III or similar enzymes in which progressively more remote regions of the target DNA are brought into range for sequencing by universal primers.

Index Entries

Sequencing repetitive DNA partial endonuclease leavage PCR 

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References

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Copyright information

© Humana Press Inc 1995

Authors and Affiliations

  • F. G. C. Abath
    • 1
  • A. A. Holder
    • 2
  1. 1.Centro de Pesquisas Aggeu Magalhaes-FIOCRUZCidade UniversitariaRecifeBrazil
  2. 2.National Institute for Medical ResearchLondonUK

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