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Applied Biochemistry and Biotechnology

, Volume 49, Issue 3, pp 281–290 | Cite as

Extraction and chemical investigation of Kulthi (Macrotylona uniflorus, Lam.) seed protein

  • B. Basak
  • U. K. Bhattacharyya
  • A. Sinhababu
  • S. Laskar
Article

Abstract

Studies have been carried out on the protein solubility profile of Kulthi (Macrotylona uniflorus, Lam.) seed in aqueous solution over various pHs and at different concentrations of NaCl, Na2SO3, CaCl2, and MgCl2 at pH 8.0. Amino acid analysis of isolated protein identified 17 amino acids, 9 of which are essential. Gel-permeation chromatography on Sephadex G-200 revealed the presence of seven components in the protein fraction. Their molecular weights were determined by two comparable standard methods. Extractable Kulthi seed proteins in salt solutions were separated electrophoretically into eight fractions whose molecular weights were found to be 186,200, 131,800, 108,400, 91,200, 53,700, 44,700, 38,000, and 27,500.

Index Entries

Macrotylona uniflorus (Dolichos biflorus) seed proteins amino acids gel filtrations SDS-PAGE 

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Copyright information

© Humana Press Inc 1994

Authors and Affiliations

  • B. Basak
    • 1
  • U. K. Bhattacharyya
    • 1
  • A. Sinhababu
    • 1
  • S. Laskar
    • 1
  1. 1.Natural Product Laboratory, Department of ChemistryUniversity of BurdwanWest BengalIndia

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