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Chinese Journal of Clinical Oncology

, Volume 1, Issue 3, pp 190–195 | Cite as

Significance of cell-free epstein-barr virus DNA in monitoring prognosis of nasopharyngeal carcinoma

  • Sumei Cao
  • Huaqing Min
  • Jinsong Gao
  • Minghuang Hong
  • Xibin Xiao
  • Chongqing Zhang
  • Xiaodong Liu
  • Ailan Zhang
  • Xiang Guo
Original Articles
  • 8 Downloads

Abstract

Objective

It has been reported that cell-free Epstein-Barr virus (EBV-DNA) in plasma was useful in diagnosing and monitoring nasopharyngeal carcinoma (NPC). The current study was designed to evaluate the significance of EBV-DNA in monitoring the prognosis of nasopharyngeal carcinoma and comparing its significance with that of plasma VCA/lgA and EA/lgA levels.

Methods

E8V -DNA, VCA/lgA, and EA/lgA levels in plasma were determined in NPC patients with different prognosis after radiotherapy, including 30 distant metastatic patients, 22 local recurrence patients and 24 individuals with remission who had been followed-up for more than 2 years after treatment. EBV-DNA was determined using a real-time quantitative PCR system, and levels of VCA/lgA and EA/lgA were measured using standard immunofluorescence. In a cohort study, the indexes were determined after different radiation periods for the 20 new cases of nasopharyngeal carcinoma.

Results

The median plasma EBV-DNA concentration was 135,100 copies/ ml (interquartile range: 5,525-1,003 750) in metastatic group, 20,500 copies/ ml (interquartile range: 0 -58,500) in the local recurrence group and 0 copies/ml (interquartile range: 0-0) in the continuous remission group (P< 0.05). The levels of VCA/lgA and EA/lgA showed no significant differences among the different groups. The high level of EBV-DNA concentration in the metastatic group was more than that in the local recurrence group. A level of 1,000,000 copies/ml of EBV DNA was an indication of distant metastasis of the NPC patients with a sensitivity of 27.3%. However, the sensitivity was 0 in the local recurrence group. For the 20 new patients, EBV -DNA concentration gradually decreased during the radiation period. Before radiation there were 32,050 copies/ml (interquartile range: 3,880-317,750), 0 copies/ml (interquartile range: 0-14 375) after a 40 Gy radiation dose and 0 copies/ml (interquartile range: 0-2940) after the radiation was finished (P< 0.05). However, the levels of VCA/lgA and EA/lgA showed no significant difference.

Conclusion

Determination of plasma cell -free EBV -DNA level is more valuable than evaluation of VCA/lgA and EA/lgA for monitoring the prognosis of NPC patients.

Keywords

nasopharyngeal carcinoma (NPC) DNA Epstein-Barr virus (EBV) polymerase chain reaction (PCR) prognosis 

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References

  1. 1.
    Min HQ. Nasopharyngeal Carcinoma. Li SL, Chief editor. Head & Neck Carcinoma. Tianjin: Tianjin Science & Technology Press. 1993:536–606.Google Scholar
  2. 2.
    Lee AW, Poo W, Law SC, et al. Staging of nasopharyngeal carcinoma: From Ho’s to the new UICC system. Int J Cancer. 1990:84:179–187.CrossRefGoogle Scholar
  3. 3.
    Hong MH, Fang JQ, Ma J, et al. Using FLF and FDM for prognostic study of nasopharyngeal carcinoma — with 411 cases of nasopharyngeal carcinoma analysis. Chin J Cancer. 1998;17: 118.Google Scholar
  4. 4.
    Chen CL, Wen WN, Chen JY, et al. Detection of Epstein — Barr virus genome in nasopharyngeal carcinoma by in situ DNA hybridization. Intervirology. 1993;36: 91–98.PubMedGoogle Scholar
  5. 5.
    Lo YM, Chan LY, Lo KW. et al. Quantitative analysis of cell -free Epstein -Barr virus DNA in plasma of patients with Nasopharyngeal Carcinoma. Cancer Res. 1999;59: 1188–1192.PubMedGoogle Scholar
  6. 6.
    Lo YM. Chan LY, Chan AT, et al. Quantitative and temporal correlation between circulating cell -free Epstein -Barr virus DNA and tumor recurrence in nasopharyngeal carcinoma. Cancer Res. 1999:59:5452–5455.PubMedGoogle Scholar
  7. 7.
    Lo YM. Chan AT. Chan LY. et al. Molecular prognostication of nasopharyngeal carcinoma by quantitative analysis of circulating Epstein-Barr virus DNA. Cancer Res. 2000; 60:6878–6881.PubMedGoogle Scholar
  8. 8.
    Cao SM, Gao JS, Liu XD, et al. Significance of plasma EBV-DNA detected by real-time quantitative PCR in diagnosing nasopharyngeal carcinoma. Chin J Cancer. 2002;2l: 328–329.Google Scholar
  9. 9.
    Min M, Hong M, Ma J, et al. A new staging system for nasopharyngeal carcinoma in China. Int J Radiat Oncol Biol Phys. 1994:30:1037–1024.PubMedGoogle Scholar
  10. 10.
    Liu YX. Detection of VCA/IgA antibody in nasopharyngeal carcinoma patients by immunolluorescence. Chin J Oncol. 1979 1:118.Google Scholar
  11. 11.
    Fang JQ. Design and analysis in diagnosing and screening. Fang JQ, Chief Editor. Medical Statistics & Computer Experiment. Shanghai: Shanghai Science & Technology Press. 1995: 224.Google Scholar
  12. 12.
    Liu MZ, Guan XX, Gao JM, et al. Relationship between EB virus VCA-IgA antibody and long-term cure of nasopharyngeal carcinoma. Chin J Cancer. 1998;17:365–367.Google Scholar
  13. 13.
    Wyatt De, Brooker DS, Connolly JH, et al. Prognostic value of Epstein -Ban virus serology in patients with nasopharyngeal carcinoma. J Infect. 1993:26:171–175.PubMedCrossRefGoogle Scholar
  14. 14.
    Sam CK, Abu-Samah AJ, Prasad U. IgA/VCA as a followup marker in the monitoring of imopharyngeai carcinoma. Eur J Surg Oneol.1994;20:561–564.Google Scholar
  15. 15.
    Peng PJ, Zhao C, Zhang L, et al. Relationship between the kinetics of plasma EBV-DNA levels and clinical response during treatment in patients with nasopharyngeal carcinoma. Chin J Cancer. 2002;21: 817–824.Google Scholar

Copyright information

© Chinese Anti-Cancer Association and Springer 2004

Authors and Affiliations

  • Sumei Cao
    • 1
  • Huaqing Min
    • 1
  • Jinsong Gao
    • 2
  • Minghuang Hong
    • 1
  • Xibin Xiao
    • 3
  • Chongqing Zhang
    • 3
  • Xiaodong Liu
    • 2
  • Ailan Zhang
    • 1
  • Xiang Guo
    • 1
  1. 1.Deptment of Nasopharyngeal CarcinomaCancer Center of Sun Yat-sen UniversityGuangzhouChina
  2. 2.Da’an Gene Diagnostic CenterSun Yat-sen UniversityGuangzhouChina
  3. 3.Laboratory, Cancer CenterSun Yat-sen UniversityGuangzhouChina

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