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Molecular Neurobiology

, Volume 6, Issue 2–3, pp 179–190 | Cite as

Axon-myelin transfer of phospholipids and phospholipid precursors

Labeling of myelin phosphoinositides through axonal transport
  • Robert W. Ledeen
  • Francis Golly
  • James E. Haley
Article

Abstract

Previous studies have provided evidence for axon-to-myelin transfer of intact lipids and lipid precursors for reutilization by myelin enzymes. Several of the lipid constituents of myelin showed significant contralateral/ipsilateral ratios of incorporated radioactivity, indicative of axonal origin, whereas proteins and certain other lipids did not participate in this transfer-reutilization process. The present study will examine the labeling of myelin phosphoinositides by this pathway. Both32PO4 and [3H]inositol were injected monocularly into 7-9-wk-old rabbits and myelin was isolated 7 or 21 days later from pooled optic tracts and superior colliculi. In total lipids32P counts of the isolated myelin samples showed significant contralateral/ipsilateral ratios as well as increasing magnitude of contralateral-ipsilateral differences during the time interval. Thin-layer chromatographic isolation of the myelin phosphoinositides revealed significant32P-labeling of these species, with PIP and PIP2 showing time-related increases. This resembled the labeling pattern of the major phospholipids from rabbit optic system myelin in a previous study and suggested incorporation of axon-derived phosphate by myelin-associated enzymes. The32P label in PI, on the other hand, remained constant between 7 and 21 days, sugesting transfer of intact lipid. This was supported by the labeling pattern with [3H]inositol, which also showed no increase over time for PI. These results suggest axon-myelin transfer of intact PI followed by myelin-localized incorporation of axon-derived phosphate groups into PIP and PIP2. The general topic of axon-myelin transfer of phospholipids and phospholipid precursors is reviewed.

Index Entries

Myelin myelin phospholipids: myelin phosphoinositides axonal-transport axon-myelin transfer 

Abbreaviations

PI

phosphatidylinositol

PIP

phosphatidylinositol 4-monophosphate

PIP2

phosphatidylinositol 4,5-bisphosphate

CL

contralateral

IL

ipsilateral

EM

electron microscope

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Copyright information

© The Humana Press, Inc 1992

Authors and Affiliations

  • Robert W. Ledeen
    • 1
  • Francis Golly
    • 1
  • James E. Haley
    • 1
  1. 1.Departments of Neurology and BiochemistryAlbert Einstein College of MedicineBronx

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