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Axon-myelin transfer of phospholipids and phospholipid precursors

Labeling of myelin phosphoinositides through axonal transport

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An Erratum to this article was published on 01 December 1992

Abstract

Previous studies have provided evidence for axon-to-myelin transfer of intact lipids and lipid precursors for reutilization by myelin enzymes. Several of the lipid constituents of myelin showed significant contralateral/ipsilateral ratios of incorporated radioactivity, indicative of axonal origin, whereas proteins and certain other lipids did not participate in this transfer-reutilization process. The present study will examine the labeling of myelin phosphoinositides by this pathway. Both32PO4 and [3H]inositol were injected monocularly into 7-9-wk-old rabbits and myelin was isolated 7 or 21 days later from pooled optic tracts and superior colliculi. In total lipids32P counts of the isolated myelin samples showed significant contralateral/ipsilateral ratios as well as increasing magnitude of contralateral-ipsilateral differences during the time interval. Thin-layer chromatographic isolation of the myelin phosphoinositides revealed significant32P-labeling of these species, with PIP and PIP2 showing time-related increases. This resembled the labeling pattern of the major phospholipids from rabbit optic system myelin in a previous study and suggested incorporation of axon-derived phosphate by myelin-associated enzymes. The32P label in PI, on the other hand, remained constant between 7 and 21 days, sugesting transfer of intact lipid. This was supported by the labeling pattern with [3H]inositol, which also showed no increase over time for PI. These results suggest axon-myelin transfer of intact PI followed by myelin-localized incorporation of axon-derived phosphate groups into PIP and PIP2. The general topic of axon-myelin transfer of phospholipids and phospholipid precursors is reviewed.

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Abbreviations

PI:

phosphatidylinositol

PIP:

phosphatidylinositol 4-monophosphate

PIP2 :

phosphatidylinositol 4,5-bisphosphate

CL:

contralateral

IL:

ipsilateral

EM:

electron microscope

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Authors and Affiliations

  1. Departments of Neurology and Biochemistry, Albert Einstein College of Medicine, 1300 Morris Park Avenue, 10461, Bronx, NY

    Robert W. Ledeen, Francis Golly & James E. Haley

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  1. Robert W. Ledeen
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  2. Francis Golly
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An erratum to this article is available at http://dx.doi.org/10.1007/BF02757948.

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Ledeen, R.W., Golly, F. & Haley, J.E. Axon-myelin transfer of phospholipids and phospholipid precursors. Mol Neurobiol 6, 179–190 (1992). https://doi.org/10.1007/BF02780551

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