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Gastroenterologia Japonica

, Volume 25, Supplement 2, pp 72–76 | Cite as

Cloning of hepatitis C virus genomes and their properties

  • Terukatsu Arima
  • Hiroyuki Shimomura
  • Takeyuki Nakajima
  • Kouichi Kanai
  • Hideo Nagashima
  • Takao Tsuji
Proceeding of the International Symposium on Biology of Hepatitis B Virus and Non-A Non-B Hepatitis Virus
  • 8 Downloads

Summary

A random primed lambda gt11-cDNA library was constructed from donors plasma presumably infected by blood-borne non-A, non-B hepatitis (hepatitis C:HC) agent and immunoscreened with serum pooled from patients with acute or chronic HC. Twelve lambda gtll-cDNA clones were isolated that was shown to encode antigens associated specifically with HC infection in Japan as well as in USA. Of these two, as well as another clone which is specific only to Japanese HC infection, have unique nucleotide sequences and were extensively studied. They are not derived from host DNA and have no homology to the sequences of known human viruses including hepatitis A, B and D viruses, Ebstein-Barr virus, coxsackievirus, immunodeficiency virus type 1 or Japanese encephalitis virus. These results suggest that they are derived from the genome of HC agent(s). In addition, of these, one clone seems to encode epitopes derived from both the core and the surface polypeptides of the agent.

Key words

cloning hepatitis C lambda gt11 library RNA virus 

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Copyright information

© The Japanese Society of Gastroenlerology 1990

Authors and Affiliations

  • Terukatsu Arima
    • 1
  • Hiroyuki Shimomura
    • 1
  • Takeyuki Nakajima
    • 2
  • Kouichi Kanai
    • 3
  • Hideo Nagashima
    • 4
  • Takao Tsuji
    • 1
  1. 1.First Department of Internal MedicineOkayama University Medical SchoolOkayamaJapan
  2. 2.Second Department of Internal MedicineHamamatsu University School of MedicineHamamatsuJapan
  3. 3.Toshiba Central HospitalTokyoJapan
  4. 4.Kawatetsu Mizushima HospitalOkayamaJapan

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