Summary
A random primed lambda gt11-cDNA library was constructed from donors plasma presumably infected by blood-borne non-A, non-B hepatitis (hepatitis C:HC) agent and immunoscreened with serum pooled from patients with acute or chronic HC. Twelve lambda gtll-cDNA clones were isolated that was shown to encode antigens associated specifically with HC infection in Japan as well as in USA. Of these two, as well as another clone which is specific only to Japanese HC infection, have unique nucleotide sequences and were extensively studied. They are not derived from host DNA and have no homology to the sequences of known human viruses including hepatitis A, B and D viruses, Ebstein-Barr virus, coxsackievirus, immunodeficiency virus type 1 or Japanese encephalitis virus. These results suggest that they are derived from the genome of HC agent(s). In addition, of these, one clone seems to encode epitopes derived from both the core and the surface polypeptides of the agent.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Feinstone SM, Kapikian AZ, Purcell PH, et al: Transfusion-assosiated hepatitis not due to viral hepatitis type A or B. N Engl J Med 1975;292:767–770
Dienstag JL, Alter HJ: Non-A, non-B hepatitis: Evolving epidemiologic and clinical perspective. Seminars in Liver disease 1986;6:67–81
Bradley DW: The agents of non-A, non-B viral hepatitis. J Virol Meth 1985;10:307–319
Bradley DW, Maynard JE; Etiology and natural history of posttransfusion and enterically-transmitted non-A, non-B hepatitis. Seminars in Liver Disease 1986;6:56–66
Hakim AA: Isolation and functional property of mRNA coding for hepatitis A, B, and non-A, non-B viral particles from human sera. Naturwissenschaften 1986;73:45–47
Linke HK, Amann CW, Schlauder GG: Non-A, non-B hepatitis infection does not result in the production of abundant poly-A-containing messenger RNAs. In: Zuckerman AJ, ed. Viral Hepatitis and Liver Disease. Alan R.Liss, Inc, New York. 1988:564–567
Arima T, Shimomura H, Tsuji T, et al: Serum RNA associated with blood-transmitted non-A, non-B hepatitis. Hepatology 1988;8:1275(abstract).
Choo Q-L, Kuo G, Weiner AJ, et al: Isolation of a cDNA clone derived from a blood-borne non-A, non-B hepatitis genome. Science 1989;244:359–361
Chomczynski P, Sacchi N: Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 1987;162:156–159
Maxwell IH, Maxwell F, Hahn WE: Removal of RNase activity from DNase by affinity chromatography on agarose-coupled aminophenyl-phosphoryl-uridine 2,3 -phosphate. Nucleic Acids Res 1977;4:241–246
Maniatis T, Fritsch EF, Sambrook J: Agarose gel electrophoresis. In: Maniatis T, Fritsch EF, Sambrook J, eds Molecular Cloning. Cold Spring Harbor Laboratory: Cold Spring Harbor, 1982:150–172
Davis LG, Dibner MD, Battey JF: Preparation of DNA from eukaryotic cells In: Davis LG, Dibner MD, Battey JF, eds. Basic Methods in Molecular Biology. Elsevier Science Publishing Co., Inc., New York: 1986;42–65
Davis LG, Dibner MD, Battey JF: Preparation of DNA from lambda gt10 and lambda gt11 cDNA clones, ibidIn: 1986:216–273
Hattori M, Sakaki Y: Dideoxy sequencing method using denatured plasmid templates. Anal Biochem 1986;152:232–238
Sumiyoshi H, Mori C, Fuke I, et al: Complete nucleotide sequence of the Japanese encephalitis virus genome RNA. Virology 1987;161:497–510
Maniatis T, Fritsch EF, Sambrook J: Vectors that express cloned DNA in Escherichia coli. In: Maniatis T, Fritsch EF, Sambrook J, eds Molecular Cloning. Cold Spring Harbor Laboratory: Cold Spring Harbor, 1982:403–433
Davis LG, Dibner MD, Battey JF: Western blot analysis. In: Davis LG, Dibner MD, Battey JF, eds. Basic Methods in Molecular Biology. Elsevier Science Publishing Co., Inc, New York. 1986:311–314
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Arima, T., Shimomura, H., Nakajima, T. et al. Cloning of hepatitis C virus genomes and their properties. Gastroenterol Jpn 25 (Suppl 2), 72–76 (1990). https://doi.org/10.1007/BF02779932
Issue Date:
DOI: https://doi.org/10.1007/BF02779932