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Gastroenterologia Japonica

, Volume 25, Supplement 2, pp 43–48 | Cite as

Integration of hepatitis virus DNA near c-myc in woodchuck hepatocellular carcinoma

  • Tsuey-ying Hsu
  • Geneviéve Fourel
  • Jeanne Etiemble
  • Pierre Tiollais
  • Marie-Annick Buendia
Proceeding of the International Symposium on Biology of Hepatitis B Virus and Non-A Non-B Hepatitis Virus

Summary

A total of 33 hepatocellular carcinomas, induced in woodchucks by chronic infection with woodchuck hepatitis virus (WHV), a virus closely related to the human hepatitis B virus, were analyzed for the state of viral DNA, the expression of viral genes and of different cellular proto-oncogenes. Low levels of viral replication and presence of integrated viral forms including sequences of the enhancer element, appeared as a general rule in these tumors. Enhanced expression of one or more of the nuclear protooncogenes: c-myc, N-myc, c-fos, c-jun and jun-B was frequently observed. In two hepatomas, elevated expression and allelic alterations of c-myc were subsequent to integration of WHV DNA near the c-myc coding domain. The viral strategy for insertional activation of c-myc in these tumors appeared basically identical to that of mammalian retroviruses in T-cell lymphomas of mice and rats. Whether insertional mutagenesis of different oncogenes may be more generally linked to liver oncogenesis induced by WHV and hepatitis B viruses remains to be determined.

Key words

c-myc proto-oncogene hepatitis B virus hepatocellular carcinoma insertional mutagenesis woodchuck 

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Copyright information

© The Japanese Society of Gastroenlerology 1990

Authors and Affiliations

  • Tsuey-ying Hsu
    • 1
  • Geneviéve Fourel
    • 1
  • Jeanne Etiemble
    • 1
  • Pierre Tiollais
    • 1
  • Marie-Annick Buendia
    • 1
  1. 1.Unité de Recombinaison et Expression Génétique (INSERM U.163, CNRS UA.271)Institut PasteurParis Cédex 15France

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