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Enzymatic synthesis of butyryl-rutin ester in organic solvents and its cytogenetic effects in mammalian cells in culture

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Abstract

Enzymic acylation of a flavonoid, rutin, with trichloroethylbutyrate (TCEB) has been performed by subtilisin protease in anhydrous pyridine solution. The addition of a hydrophobic compound on rutin is expected to change the hydrophilic/hydrophobic balance of the molecule, giving new properties to this compound.

This work aimed at investigating the various cytological properties of the rutin-ester and compared them with those of the native molecule. No difference in the levels of sister chromosomes exchange (SCE) between rutin and rutin-ester treated cells at doses varying from 25 to 200 μg/mL was found. On the contrary impressive difference in the induced frequency of micronuclei (MN) between rutin and rutin ester treated cells was observed, for example, at a dose of 100 μg/mL of rutin were 3.5% MN counted, whereas for a similar dose treatment with rutin-ester a frequency of 8% of MN was found.

The fact that rutin-ester is causing significantly higher levels of MN than the rutin alone can be considered as a manifestation of a higher action of the agent on the chromosome owing to its easier penetration in to the cell after its esterification.

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Authors and Affiliations

  1. Chemical Engineering Dept., Division IV, National Technical University of Athens, 9 Iroon Polytechniou, Zografou Campus, 15700, Athens, Greece

    G. Kodelia & F. N. Kolisis

  2. School of Education, Aristelian University of Thessaloniki, Greece

    K. Athanasiou

Authors
  1. G. Kodelia
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  2. K. Athanasiou
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  3. F. N. Kolisis
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Kodelia, G., Athanasiou, K. & Kolisis, F.N. Enzymatic synthesis of butyryl-rutin ester in organic solvents and its cytogenetic effects in mammalian cells in culture. Appl Biochem Biotechnol 44, 205–212 (1994). https://doi.org/10.1007/BF02779657

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  • DOI: https://doi.org/10.1007/BF02779657

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