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Applied Biochemistry and Biotechnology

, Volume 6, Issue 2, pp 153–166 | Cite as

Influence of urea and organic solvents on the activity of immobilizedmyo-inositol-1-phosphate synthase containing active, self-regenerating coenzyme (NAD+) on the same matrix

  • F. Pittner
Article

Abstract

myo-Inositol-1-phosphate synthase (EC 5.5.1.4.) from rat testes, an NAD+-containing enzyme that convertsd-glucose 6-phosphate to 1l-myo-inositol-1-phosphate was immobilized together with its cofactor and bovine serum albumin by crosslinking with glutaraldehyde at pH 4.5. The cofactor is reduced and reoxidized during the reaction cycle, thus forming a self-regenerating system with respect to the cofactor. The behavior of this immobilized enzyme/cofactor system in presence of organic solvents and urea and the activating effect of these compounds on the enzymatic activity were studied and discussed in the paper.

Index Entries

myo-Inositol-1-phosphate NAD immobilized enzyme organic solvent, effect on immobilized enzyme activity urea, effect on immobilized enzyme activity coenzyme NAD+, self-regenerating regenerating, coenzyme NAD+ synthase,myo-inositol-1-phosphate 

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References

  1. 1.
    Pittner, F. (1981),Appl Biochem. Biotechnol. 6, 85.Google Scholar
  2. 2.
    Pittner, F., Fried, W., and Hoffman-Ostenhof, O. (1974),Hoppe Seyler’s Z. Physiol. Chem. 355, 222–224.Google Scholar
  3. 3.
    Pittner, F., and Hoffmann-Ostenhof, O. (1979),Mol. Cell. Biochem. 28, (1–3), 23–26.Google Scholar
  4. 4.
    Barnett, J. E. G., Brice R. E., and Corina, D. L. (1970),Biochem. J. 119, 183–186.Google Scholar
  5. 5.
    Jacobs, S. (1959),Nature 183, 262.CrossRefGoogle Scholar
  6. 6.
    Hauska, G., and Hoffman-Ostenhof, O. (1967),Hoppe Seyler’s Z. Physiol. Chem. 348, 1558–1559.Google Scholar
  7. 7.
    Tanford, C., Bunville L. G., and Nozaki Y. (1959),J. Am. Chem. Soc. 81, 4032–4036.CrossRefGoogle Scholar
  8. 8.
    Tanford, C., and Taggart, V. G. (1961),J. Am. Chem. Soc. 83, 1634–1638.CrossRefGoogle Scholar
  9. 9.
    Pittner, F., and Hoffmann-Ostenhof, O. (1979),Mol. Cell. Biochem. 28, 1–3.CrossRefGoogle Scholar
  10. 10.
    Tanford, C. (1968),Adv. Protein Chem. 23, 122–282.Google Scholar

Copyright information

© The Humana Press Inc 1981

Authors and Affiliations

  • F. Pittner
    • 1
    • 2
  1. 1.Institut für Allgemeine Biochemie der Universität WienWienAustria
  2. 2.Ludwig-Boltzmann-Forschungsstelle für BiochemieWienAustria

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