Abstract
The kinetics of oxidative phenol degradation with microbial cellsCandida tropicalis, immobilized in a polyacrylamide and polymethacrylamide matrix, were mathematically simulated assuming zero-order and Michaelis-Menten rate equations.
For zero-order kinetics an expanded equation for catalytic effectiveness as a function of the Thiele modulus, Biot number, and partition coefficients was derived and compared with numerical solutions for Michaelis-Menten kinetics. Errors with regard to the zero-order approximation become negligible ifc o/K M >2.
Experimentally determined catalyst activities as a function of particle size and cell concentration were compared to calculated ones. Additional experiments to determine the diffusion and oxygen consumption ratios have been carried out in an effort to resolve the physical parameters to be used in the above mentioned calculations.
Furthermore, experiments on cell growth during reincubation with nutrients and oxygen are reported; an increase in activity up to a factor of ten was observed. These experiments demonstrate that the microbial cells are entrapped in the polymer matrix in the living state.
Similar content being viewed by others
References
Klein, J., and Schara, P. (1979),J. Solid Phase Biochem. 5, 61.
Kasche, V., Lundquist, H., Bergman, R., and Axén, R. (1971),Biochem. Biophys. Res. Commun. 45, 615.
Korus, R., and O’Driscoll, K. F.,Canad. J. Chem. Eng. 52, 5 (1974).
Vieth, W. R., Venkatasubramanian, K., Constantinides, A., and Davidson, B. (1976),Appl. Biochem. Bioeng. 1, 221.
Greenfield, P. F., Kittrell, J. R., and Laurence, R. L. (1976),Anal. Biochem. 65, 109.
Buchholz, K., and Reuss, M. (1977),Chimia 31, 27.
Klein, J., Hackel, U., Schara, P., Washausen, P., and Wagner, F. (1976),Abstr. 5th Intern. Ferment. Symp. Berlin., 295.
Klein, J., Hackel, U., Schara, P., Washausen, P., Wagner, F. and Martin, C. K. A. (1978),Enzyme Eng. 4, 339.
Satterfield, C. N. (1970),Mass Transfer in Heterogeneous Catalysis, Cambridge, Mass.
Aris, R. (1975),The Mathematical Theory of Diffusion and Reaction in Permeable Catalysts, vol. 1, Clarendon Press, Oxford.
Washausen, P. (1975), Diplomarbeit, Institut f. chemische Technologie der Technischen Univerität Braunschweig.
Nei, N., Enatsu, T., and Terui, G. (1973),J. Ferm. Techn. 51, 1.
Krasuk, J. H., and Smith, J. M. (1965),Ind. Eng. Chem. Fund. 4, 102.
Moo-Young, M., and Kobayashi, T. (1972),Can. J. Chem. Eng.,50, 162.
Bischoff, K. B. (1965),AIChEJ.,11, 351.
Na, H. S., and Na, T. Y. (1970),Mathem. Biosci. 6, 25.
Fink, D. J., Na, T. J., and Schultz, J. S. (1973),Biotech. Bioeng. 15, 879.
Wadiak, D. T., and Carbonell, R. G. (1975),Biotech. Bioeng. 17, 1761.
Daynes, H. A. (1920).Proc. Roy. Soc. (London) A97, 286.
Neujahr, H. J., Lindsjö, S., and Varga, J. M. (1974),Antonie van Leuwenhoek 40, 209.
Klein, J., and Hackel, U. (1979),ACS Symp. Series 106, 101.
Erdös, E., and Nyvld, J. A. (1958),Collect. Czechoslov. Chem. Comm. 23, 579.
White, M. L., and Dorion, G. H. (1961),J. Polym. Sci. 55, 731.
Landolt-Börnstein, Zahlenwerte und Funktionen, 6. Auflage, Vol. II (1969).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Klein, J., Schara, P. Entrapment of living microbial cells in covalent polymeric networks. Appl Biochem Biotechnol 6, 91–117 (1981). https://doi.org/10.1007/BF02779243
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02779243