Applied Biochemistry and Biotechnology

, Volume 6, Issue 2, pp 85–90 | Cite as

Immobilization ofmyo-inositol-1-phosphate synthase containing active, self-regenerating coenzyme (NAD+) on the same matrix

  • F. Pittner


myo-Inositol-1-phosphate synthase (EC from rat testes, an NAD+-containing enzyme, which convertsd-glucose 6-phosphate to 1l-myo-inositol 1-phosphate, could be immobilized together with its cofactor and bovine serum albumin by crosslinking with glutaraldehyde at pH 4.5. The enzyme bound to the gel showed a specific activity of 5.6% of that of the native enzyme, but the activity could be increased to 21% by pretreatment with urea.

Index Entries

Glutaraldehyde crosslinking immobilized inositol phosphate synthase bound NAD synthase immobilized inositol phosphate NAD, immobilization of bound 


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Copyright information

© The Humana Press Inc 1981

Authors and Affiliations

  • F. Pittner
    • 1
    • 2
  1. 1.Institut für Allgemeine Biochemie der Universität WienWienAustria
  2. 2.Ludwig-Boltzmann-Forschungsstelle für BiochemieWienAustria

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