Gastroenterologia Japonica

, Volume 28, Issue 2, pp 242–248 | Cite as

Effects of cytokines on HLA class I antigen expression on Huh6 and HB611 cells

  • Takahiro Towata
  • Norio Hayashi
  • Kazuhiro Katayama
  • Tetsuo Takehara
  • Eiji Miyoshi
  • Yuko Kawanishi
  • Keiji Ueda
  • Akinori Kasahara
  • Hideyuki Fusamoto
  • Takenobu Kamada
Liver, Pancereas, and Biliary Tract


This study investigated the expression of HLA class I antigens on Huh6 and HB611 cells induced by interferon (IFN)-α, IFN-γ, tumor necrosis factor (TNF)-α and interleukin (IL)-lβ. All of these cytokines induced the antigens on both cells in a dose-dependent manner, with IFNs inducing much more expression than TNF-α or IL-1β. We have already reported that protein kinase C (PKC) is involved in the antigen expression induced by IFN-γ on Huh6 cells. The antigen expression induced by IFN-α was also blocked by a PKC inhibitor, H-7. However, the antigen expression by TNF-α or IL-1β was not inhibited by H-7, by a protein kinase A inhibitor, HA1004, nor by a calmodulin antagonist, W-7. These results suggested that PKC, Ca2+-calmodulin, and cAMP are not involved in the induction of HLA class I antigens on both cells by TNF-a and IL-1β. We concluded that TNF-a and IL-1β induced much less expression of HLA class I antigens on both cells than IFNs and that this might be because the signaling pathway by TNF-α and IL-1β differed from that by IFNs.

Key words

interferon tumor necrosis factor interleukin-1 HLA class I antigen Huh6 HB611 


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Copyright information

© The Japanese Society of Gastroenterology 1993

Authors and Affiliations

  • Takahiro Towata
    • 1
  • Norio Hayashi
    • 1
  • Kazuhiro Katayama
    • 1
  • Tetsuo Takehara
    • 1
  • Eiji Miyoshi
    • 1
  • Yuko Kawanishi
    • 1
  • Keiji Ueda
    • 1
  • Akinori Kasahara
    • 1
  • Hideyuki Fusamoto
    • 1
  • Takenobu Kamada
    • 1
  1. 1.First Department of MedicineOsaka University Medical SchoolOsakaJapan

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