Applied Biochemistry and Biotechnology

, Volume 17, Issue 1–3, pp 357–369 | Cite as

Production of ethanol from sugar cane bagasse hemicellulose hydrolyzate byPichia stipitis

Scientific note
  • Carina Van Zyl
  • Bernard A. Prior
  • James C. Du Preez
Session 2 Scientific Notes


The ability ofPichia stipitis to fermentd-xylose andd-glucose in the acid-hydrolyzed hemicellulose component of sugar cane bagasse depends on the alkali used to neutralize the hydrolyzate to pH 6.5. With NH4OH and NaOH no fermentation occurred, whereas neutralization with Ca(OH)2 gave the best results (Qpmax=0.25 g/L-h; Yp/s =0.38 g/g sugar). However, the volumetric productivity was still considerably less than observed in a semisynthetic medium with a sugar composition similar to the hydrolyzate. L-arabinose was not fermented but assimilated. Sequential neutralization methods failed to improve the fermentation. Acetic acid and lignin derivatives present in the hydrolyzate were major components that inhibited the fermentation.

Index Entries

Hemicellulose hydrolyzate d-xylose fermentation ethanol production inhibitors Pichia stipitis neutralization techniques 


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Copyright information

© The Humana Press Inc 1988

Authors and Affiliations

  • Carina Van Zyl
    • 1
  • Bernard A. Prior
    • 1
  • James C. Du Preez
    • 1
  1. 1.Department of MicrobiologyUniversity of the Orange Free StateBloemfonteinSouth Africa

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