Production of ethanol from sugar cane bagasse hemicellulose hydrolyzate byPichia stipitis
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The ability ofPichia stipitis to fermentd-xylose andd-glucose in the acid-hydrolyzed hemicellulose component of sugar cane bagasse depends on the alkali used to neutralize the hydrolyzate to pH 6.5. With NH4OH and NaOH no fermentation occurred, whereas neutralization with Ca(OH)2 gave the best results (Qpmax=0.25 g/L-h; Yp/s =0.38 g/g sugar). However, the volumetric productivity was still considerably less than observed in a semisynthetic medium with a sugar composition similar to the hydrolyzate. L-arabinose was not fermented but assimilated. Sequential neutralization methods failed to improve the fermentation. Acetic acid and lignin derivatives present in the hydrolyzate were major components that inhibited the fermentation.
Index EntriesHemicellulose hydrolyzate d-xylose fermentation ethanol production inhibitors Pichia stipitis neutralization techniques
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- 1.Dekker, R. F. H., and Lindner, W. A. (1979),S. Afr. J. Sci. 75, 65.Google Scholar
- 3.Lee, Y. Y., and McCaskey, T. A. (1983),Tappi J. 66, 102.Google Scholar
- 5.Hajny, G. I. (1981),Biological utilization of wood for production of chemicals and foodstuffs. US Dept. Agric. For. Serv. For. Prod. Lab., Madison, WI, Research Paper FPL 385.Google Scholar
- 6.Magee, R. J., and Kosaric, N. (1985),Adv. Biochem. Eng./Biotechnol. 32, 61.Google Scholar
- 11.Wickerham, L. J. (1951),Taxonomy of yeasts. US Dept. Agric., Washington, DC, Tech. Bull. No. 1029.Google Scholar
- 12.Strickland, R. C., and Beck, M. J. (1984), Effective pretreatments and neutralization methods for ethanol production from acid-catalyzed hardwood hydrolyzates usingPachysolen tannophilus.Proc. 6th Int. Symp. Alcohol Fuels Technol., Ottawa, Canada, vol. 2, pp. 220–226.Google Scholar
- 14.Gong, C-S., and Tsao, G. T. (1982), Production of ethanol from biomass hemicellulose hydrolyzate by yeasts.Proc. Natl. Meet. Am. Inst. Chem. Eng., Orlando, Florida, Paper No. 43, pp. 1–17.Google Scholar
- 16.Weast, R. C., Selby, S. M., and Hodgeman, C. D., eds. (1965),Handbook of Chemistry and Physics, 46th ed., Chemical Rubber Co., Cleveland, OH, p. D-79.Google Scholar
- 17.Vogel, A. I. (1961),A Textbook of Quantitative Inorganic Analysis, 3rd ed., Longmans, Green & Co. Ltd., London, pp. 1144–1145.Google Scholar
- 21.Noller, C. R. (1958),Textbook of Organic Chemistry, Saunders, Philadelphia, PA, pp. 457–460.Google Scholar