Biological Trace Element Research

, Volume 63, Issue 3, pp 205–212 | Cite as

Effects of low-molecular-weight aluminum complexes on brain tissue calcium homeostasis

  • Leopold J. Anghileri
  • Pierre Thouvenot
  • Alain Bertrand


The in vitro effects of low-molecular-weight aluminum complexes (citrate, lactate, and ATP complex) on the Ca2+ uptake and aluminum-induced lipid peroxidation of brain tissue show that the modification of the calcium homeostasis is determined by the nature of the ligand and that there is no correlation between the aluminum-induced lipid peroxidation and the Ca2+ uptake. The same characteristics have been shown by a similar study performed with Ehrlich carcinoma cells. The electrophoretic analyses of the aluminum lactate-albumin and aluminum lactate-ATP interactions indicate an aluminum transfer from the lactate to the albumin and ATP ligands. The increased Ca2+ uptake when ATP is present in the incubation medium with aluminum citrate and aluminum lactate corroborates the suggested mediator role of ATP in cellular calcium homeostasis modification induced by iron.

Index Entries

Brain calcium homeostasis low-molecular-weight aluminum complexes 


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  1. 1.
    L. J. Anghileri, Effects of Fe3+-tumor cell interaction on Ca2+-uptake by Ehrlich ascites tumor cells,Cell Calcium 12, 371–374 (1991).PubMedCrossRefGoogle Scholar
  2. 2.
    L. J. Anghileri, Ehrlich tumor cells: Ca2+-uptake modification by aluminum lactate,Cell Calcium 13, 277–279 (1992).PubMedCrossRefGoogle Scholar
  3. 3.
    L. J. Anghileri, P. Maincent, A. Cordova Martinez, and P. Thouvenot, The role of ATP as a mediator in the action of iron complexes on cellular calcium homeostasis.Biol. Trace Element Res. 46, 103–111 (1994).CrossRefGoogle Scholar
  4. 4.
    L. J. Anghileri, P. Thouvenot, and A. Bertrand, Effects of iron complexes on brain calcium homeostasis,Ann. Clin. Lab. Sci. 27, 210–215 (1997).PubMedGoogle Scholar
  5. 5.
    J. M. C. Gutteridge, Ferrous ion-EDTA-stimulated phospholipid peroxidation,Biochem. J. 224, 697–701 (1984).PubMedGoogle Scholar
  6. 6.
    A. D. Horton and P. F. Thomason, Ion exchange—spectrophotometric determination of aluminum,Anal. Chem. 28, 1326–1329 (1956).CrossRefGoogle Scholar
  7. 7.
    J. E. Gorman and F. M. Clydesdale, Thermodynamic and kinetic stability constants of selected carboxylic acids and iron,J. Food Sci. 49, 500–504 (1984).CrossRefGoogle Scholar
  8. 8.
    L. J. Anghileri, P. Maincent, A. Cordova Martinez, and J. Escanero, Effects of albumin and adenosine phosphates on iron transfer from ferric lactate,Biol. Trace Element Res. 40, 83–88 (1994).Google Scholar
  9. 9.
    M. Favarato, P. Zatta, M. Perazzolo, L. Fontana, and M. Nicolini, Aluminum influence on the permeability of blood-brain barrier to (14C)sucrose in rats.Brain Res. 569, 330–335 (1992).PubMedCrossRefGoogle Scholar
  10. 10.
    R. A. Yokel, V. Lidums, P. J. McNamara, and V. Ungerstedt, Aluminum distribution into brain and liver of rats and rabbits following intravenous aluminum lactate or citrate: a microdialysis study,Toxicol. Appl. Pharmacol. 107, 153–163 (1991).PubMedCrossRefGoogle Scholar
  11. 11.
    S. K. Jonas and P. A. Riley, The effect of ligands on the uptake of iron by cells in culture.Cell Biochem. Funct. 9, 245–253 (1991).PubMedCrossRefGoogle Scholar
  12. 12.
    M. A. Burnatowska-Hledin and G. H. Mayor, The effects of aluminum loading on selected tissue calcium and magnesium concentration in rats,Biol. Trace Element Res. 6, 531–535 (1984).Google Scholar
  13. 13.
    P. R. S. Kodavanti, W. R. Mundy, H. A. Tilson and G. J. Harry, Effects of selected neuroactive compounds on calcium transporting systems in rat cerebellum and on survival of cerebellar granule cells,Toxicologist 12, 128–132 (1992).Google Scholar
  14. 14.
    L. J. Anghileri, Effects of complexed iron and aluminum on brain calcium,Neurotoxicology 13, 475–478 (1992).PubMedGoogle Scholar
  15. 15.
    L. J. Anghileri, P. Maincent, and P. Thouvenot, Modification of in vivo Ca2+-uptake by parenterally administered aluminum complexes,In Vivo 8, 237–240 (1994).PubMedGoogle Scholar
  16. 16.
    R. N. MacBurney and I. R. Neering, Neuronal calcium homeostasis,Trends Neuro-Sci. 10, 164–169 (1987).CrossRefGoogle Scholar
  17. 17.
    L. J. Anghileri, M. Heidbreder, G. Weiler, and R. Dermietzel, Liver tumors induced by 4-dimethylaminoazobenzene. Experimental basis for a chemical carcinogenesis concept,Arch. Geschwultsforsch 46, 639–646 (1976).Google Scholar
  18. 18.
    L. J. Anghileri, M. Heidbreder, G. Weiler, and R. Dermietzel, Hepatocarcinogenesis by thioacetamide: correlations of histological and biochemical changes, and possible role of cell injury,Exp. Cell Biol. 45, 34–47 (1977).PubMedGoogle Scholar
  19. 19.
    L. J. Anghileri, D. Stavrou and W. Weidenbach, Phospholipids and calcification in human intracranial tumors,Arch. Geschwultsforsch. 47, 330–334 (1977).Google Scholar
  20. 20.
    M. Yasui, T. Kihira, and K. Ota, Calcium, magnesium and aluminum concentrations in Parkinson’s disease,Neurotoxicology 13, 593–600 (1992).PubMedGoogle Scholar
  21. 21.
    E. Carafoli, Intracellular calcium homeostasis,Annu. Rev. Biochem. 56, 395–433 (1987).PubMedCrossRefGoogle Scholar
  22. 22.
    M. Deleers, Cationic atmosphere and cation competition binding at negative charged membranes. Pathological implications of aluminum,Res. Commun. Chem. Pathol. Pharmacol. 49, 277–294 (1981).Google Scholar
  23. 23.
    P. Nicotera and S. Orrenius, Calcium ions in necrotic and apoptotic cell death, inNeurodegeneration and Neuroprotection in Parkinson’s Diease, C. W. Olanov, P. Jenner, and M. Youdim, eds, Academic, London, p. 155 (1996).Google Scholar
  24. 24.
    L. J. Anghileri, P. Maincent, and P. Thouvenot, Role of lipid peroxidation in iron-induced calcium overload,Biol. Trace Element Res. 52, 163–169 (1996).CrossRefGoogle Scholar

Copyright information

© Humana Press Inc. 1998

Authors and Affiliations

  • Leopold J. Anghileri
    • 1
  • Pierre Thouvenot
    • 2
  • Alain Bertrand
    • 2
  1. 1.Biophysics LaboratoryMedicine Faculty University of NancyVandoeuvre lès NancyFrance
  2. 2.Nuclear Medicine ServiceUniversity of Nancy Medical CenterNancyFrance

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