Effect of oral aluminum and aluminum citrate on blood level and short-term tissue distribution of aluminum in the rat
- 72 Downloads
Aluminum (Al) absorption seems to be very low, but many factors can enhance it in animals and humans. In the present study, we investigated the acute effect of Na citrate on Al absorption by monitoring Al levels in blood and several tissues. For this purpose, 18 Wistar male rats were divided into 3 groups: control, Al, and Al + Na citrate. After a 14-h fasting period, animals were dosed orally with deionized water, or 2 mmol Al chloride, or 2 mmol Al chloride plus 2 mmol Na citrate. Blood samples were taken before and 1, 2, 4, and 6 h after the gavage. Al concentrations in blood, liver, tibia, kidney, and intestinal wall were determined by ICP-OES. In the Al and Al + citrate groups, Al blood concentrations peaked at 1 h and 2 h with higher levels in the Al + citrate group. Al gavage resulted in an increase in Al level in intestinal wall, but not in the other investigated tissues. Simultaneous gavage of citrate with Al significantly increased its tissue levels in tibia, kidney, and in intestinal wall. Our data show clearly that Al as chloride can be absorbed, but not well retained by the organism tissues. Furthermore, the model used in the present study is appropriate for acute studies to investigate the effect of various compounds on Al absorption in the rat.
Index EntriesAluminum citrate absorption status rat
Unable to display preview. Download preview PDF.
- 1.M. R. Wills and J. Savory, Aluminum poisoning: dialysis encephalopathy, osteomalacia, and anaemia,Lancet 2, 29–34 (1982).Google Scholar
- 5.C. N. Martyn, D. J. P. Barker, C. Osman, E. C. Harris, J. A. Edwardson, and R. F. Lacey, Geographical relation between Alzheimer’s disease and aluminum in drinking water,Lancet 9, 59–62 (1989).Google Scholar
- 6.Z. Deng, B. Tao, J. He, X. Li, and Y. Chen, Effect of tea on the metabolism of trace elements in rats. International conference of tea-quality-health, The Chinese Association of Tea, pp. 79–82 (1996).Google Scholar
- 7.J. Jagannatha and K. S. Rao, Aluminum leaching from utensils—a kinetic study,Intern. J. Food Sci. Nutr. 61, 449–456 (1995).Google Scholar
- 8.J. L. Greger, Aluminum content of the American diet,Food Technol. 9, 73–80 (1985).Google Scholar
- 9.J. A. T. Pennington, Aluminum content of foods and diets,Food Addit. Contam. 5, 161–232 (1987).Google Scholar
- 11.T. A. Zafar, C. M. Weaver, B. R. Martin, R. Flarend, and D. Elmore, Aluminum (26Al) metabolism in rats,P.S.E.B.M. 216, 81–85, 1997.Google Scholar
- 17.Y. Gélinas and J. P. Schmit, Comparisons between the inorganic content of healthy and hypertensive rat tissues by inductively coupled plasma-mass spectrometry,Bio-Metals 7, 155–162 (1994).Google Scholar
- 19.G. Testolin, D. Erba, S. Ciappellano, and G. Bermano, Influence of organic acids on aluminum absorption and storage in rat tissues,Food Addit. Contamin. 13, 21–27 (1996).Google Scholar
- 20.G. J. Fosmire, S. J. Focht, and G. E. McClearn, Genetic influences on tissue deposition of aluminum in mice,Biol. Trace Element Res. 37, 115–121 (1993).Google Scholar
- 23.P. Jouahanneau, B. Lacour, G. Raisbeck, F. Yiou, and T. Drüeke, Gastrointestinal absorption of aluminum using 26Al and accelerator mass spectrometer,Clin. Nephrol. 40, 244–248, 1993.Google Scholar