Biochemical studies on pigment granules in the liver of dubin-johnson syndrome
Dubin-Johnson pigment derived from 2 patients was successfully isolated and extracted with IN NaOH from 1,200×g, 10 minutes fraction of the liver homogenate after treatment with protease, amylase, DNase and acetone. The solubility, infrared spectra, elemental composition, lipids content and amino acids analysis of extracted Dubin-Johnson pigment were investigated. In the amount of nitrogen and hidrogen contents, Dubin-Johnson pigment was different from the melanin in a narrow sense. Lipids content was remarkably different between in Dubin-Johnson pigment and liver lipofuscin. Biochemical properties of bilifuscin show no similarity to those of Dubin-Johnson pigment, especially in nitrogen content in elemental composition.
From these results, the Dubin-Johnson pigment was considered to be a special substance due to characteristic metabolic failure in this syndrome different from melanin in a narrow sense, from hepatic lipofuscin as an age pigment and bilifuscin derived from bilirubin.
Key wordsPigment in Dubin-Johnson Syndrome
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- 2).Masuda, M., T. Takino, Y. Nonomura, K. Nakajima, K. Tadokoro, K. Hayashi, S. Tabuchi, R. Okawa and K. Ueda (1963): Dubin-Johnson syndrome—Analysis of pigment granules in the liver.—Saishin-igaku, 18, 1087–1103.Google Scholar
- 3).Nicolaus, R. (1955): Paper chromatography of the breakdown products of natural melanin and tyrosinase melanin with hydrogen peroxide.—Gazz. Chim. ital. 85, 659–664.Google Scholar