Gastroenterologia Japonica

, Volume 6, Issue 2, pp 55–60 | Cite as

Biochemical studies on pigment granules in the liver of dubin-johnson syndrome

  • T. Takino
  • Y. Nonomura
  • M. Masuda
  • S. Takino
Original Article


Dubin-Johnson pigment derived from 2 patients was successfully isolated and extracted with IN NaOH from 1,200×g, 10 minutes fraction of the liver homogenate after treatment with protease, amylase, DNase and acetone. The solubility, infrared spectra, elemental composition, lipids content and amino acids analysis of extracted Dubin-Johnson pigment were investigated. In the amount of nitrogen and hidrogen contents, Dubin-Johnson pigment was different from the melanin in a narrow sense. Lipids content was remarkably different between in Dubin-Johnson pigment and liver lipofuscin. Biochemical properties of bilifuscin show no similarity to those of Dubin-Johnson pigment, especially in nitrogen content in elemental composition.

From these results, the Dubin-Johnson pigment was considered to be a special substance due to characteristic metabolic failure in this syndrome different from melanin in a narrow sense, from hepatic lipofuscin as an age pigment and bilifuscin derived from bilirubin.

Key words

Pigment in Dubin-Johnson Syndrome 


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Copyright information

© The Japanese Society of Gastroenterology 1971

Authors and Affiliations

  • T. Takino
    • 1
  • Y. Nonomura
    • 1
  • M. Masuda
    • 1
  • S. Takino
    • 2
  1. 1.3rd Department of Internal MedicineKyoto Prefectural University of MedicineKyotoJapan
  2. 2.Biochemical DepartmentKyoto Prefectural University of MedicineKyotoJapan

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