Advertisement

Gastroenterologia Japonica

, Volume 6, Issue 2, pp 55–60 | Cite as

Biochemical studies on pigment granules in the liver of dubin-johnson syndrome

  • T. Takino
  • Y. Nonomura
  • M. Masuda
  • S. Takino
Original Article

Summary

Dubin-Johnson pigment derived from 2 patients was successfully isolated and extracted with IN NaOH from 1,200×g, 10 minutes fraction of the liver homogenate after treatment with protease, amylase, DNase and acetone. The solubility, infrared spectra, elemental composition, lipids content and amino acids analysis of extracted Dubin-Johnson pigment were investigated. In the amount of nitrogen and hidrogen contents, Dubin-Johnson pigment was different from the melanin in a narrow sense. Lipids content was remarkably different between in Dubin-Johnson pigment and liver lipofuscin. Biochemical properties of bilifuscin show no similarity to those of Dubin-Johnson pigment, especially in nitrogen content in elemental composition.

From these results, the Dubin-Johnson pigment was considered to be a special substance due to characteristic metabolic failure in this syndrome different from melanin in a narrow sense, from hepatic lipofuscin as an age pigment and bilifuscin derived from bilirubin.

Key words

Pigment in Dubin-Johnson Syndrome 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1).
    Wegmann, R., J. Caroli, J. Eteve, M. Rangier, A. Charbonnier et J. Brisbosis (1960): Etude histochimique, chimique et spectrographique du pigment anormal de la maladie de Dubin-Johnson.—Ann. Histochim. 5, 71–99.PubMedGoogle Scholar
  2. 2).
    Masuda, M., T. Takino, Y. Nonomura, K. Nakajima, K. Tadokoro, K. Hayashi, S. Tabuchi, R. Okawa and K. Ueda (1963): Dubin-Johnson syndrome—Analysis of pigment granules in the liver.—Saishin-igaku, 18, 1087–1103.Google Scholar
  3. 3).
    Nicolaus, R. (1955): Paper chromatography of the breakdown products of natural melanin and tyrosinase melanin with hydrogen peroxide.—Gazz. Chim. ital. 85, 659–664.Google Scholar
  4. 4).
    Hendley, D.D., A.S. Mildvan, M.C. Reporter & B.L. Strehler (1963):-The properties of isolated human caldiac age pigment. II. Chemical and enzymatic properties.—J. Gerontol. 18, 250–259.PubMedGoogle Scholar
  5. 5).
    Siebert, G., P.B. Diezel, K. Jahr, E. Krug, A. Schmitt, E. Grünberger u. I. Bottke (1962): Isolierung und Eigenschaften von Lipofuscin aus Herzgewede des Menschen.—Histochemie, 3, 17–45.PubMedCrossRefGoogle Scholar
  6. 6).
    Björkerud, S. (1964): Studies of lipofuscin granules of human cardiac muscle. II. Chemical analysis of the isolated granules.—Exp. Mol. Path. 3, 377–389.CrossRefGoogle Scholar
  7. 7).
    Folch, J., M. Less & G.H.S. Stanley (1957): A simple method for the isolation and purification of total lipids from animal tissue.—J. biol. Chem. 226, 497–509.PubMedGoogle Scholar

Copyright information

© The Japanese Society of Gastroenterology 1971

Authors and Affiliations

  • T. Takino
    • 1
  • Y. Nonomura
    • 1
  • M. Masuda
    • 1
  • S. Takino
    • 2
  1. 1.3rd Department of Internal MedicineKyoto Prefectural University of MedicineKyotoJapan
  2. 2.Biochemical DepartmentKyoto Prefectural University of MedicineKyotoJapan

Personalised recommendations