The use of an immobilized enzyme nylon tube reactor incorporating a four enzyme system for creatinine analysis
A single immobilized enzyme nylon tube reactor was produced incorporating a four enzyme system for the analysis of creatinine.
The enzyme activity ratios in the coupling solution used to prepare the reactor were found to be of extreme importance in governing the activity of the latter.
The reactor was incorporated into a continuous flow analysis system used to assay creatinine in urine samples and the results were correlated with a manual technique employing the same enzyme system in solution.
The precision, correlation, high specificity, simplicity, and speed of the analysis were concluded to be factors in favor of the method's suitability for urine creatinine determinations.
Index EntriesImmobilized enzyme reactor, for creatinine analysis reactor, creatinine analysis by immobilized enzyme nylon tube reactor, immobilized enzyme enzyme reactor, creatinine analysis using immobilized creatinine analysis, immobilized enzyme reactor for
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- 1.Jaffé, M. (1886),Z. Physiol. Chem. 10, 391.Google Scholar
- 2.Swain, R. R., and Briggs, S. L. (1977),Clin. Chem. 23, 1340.Google Scholar
- 3.Daugherty, N. A., Hammond, K. B., and Osberg, I. M. (1978),Clin. Chem. 24, 392.Google Scholar
- 5.Young, D. S., Pestaner, L. C., and Gibberman, V. (1975),Clin. Chem. 21, 286D.Google Scholar
- 6.Fabiny, D. L., and Ertingshausen, G. (1971),Clin. Chem. 17, 696.Google Scholar
- 7.Lustgarten, J. A., and Wenk, R. E. (1972),Clin. Chem. 18, 1419.Google Scholar
- 8.Bowers, L. D. (1980),Clin. Chem. 26, 551.Google Scholar
- 9.Bowers, L. D., and Wong, E. T. (1980),Clin. Chem. 26, 555.Google Scholar
- 10.Soldin, S. J., and Hill, G. J. (1978),Clin. Chem. 24, 747.Google Scholar
- 11.Spierto, R. W., MacNeil, M. L., Culbreth, P., et al. (1980),Clin. Chem. 26, 286.Google Scholar
- 13.Moss, G. A., Bondar, R. J. L., and Buzzelli, D. M. (1975),Clin. Chem. 21, 1422.Google Scholar
- 14.Jaynes, P. K., Feld, R. D., and Johnson, G. (1982),Clin. Chem. 28, 114.Google Scholar
- 17.Meerwin, H. (1966),Org. Synth. 46, 120.Google Scholar
- 18.Morris, D. L., Campbell, J., and Hornby, W. E. (1975),Biochem. J. 147, 593.Google Scholar
- 19.Noy, G. A. (1979), PhD Thesis. The Use of Immobilized Enzymes in Continuous Flow Analysis.Google Scholar
- 20.Broughton, P. M. J., Gowenlock, A. H., McCormack, J. J., and Neill, D. W., (1974),Ann. Clin. Biochem. 11, 207.Google Scholar
- 21.Nernst, W. Z. (1904),Z. Phys. Chem. 47, 52.Google Scholar
- 22.Tonks, D. B. (1963),Clin. Chem. 9, 217.Google Scholar