Abstract
For continuous production of cephalexin, whole cells ofXanthomonas citri were immobilized by entrapment in polyacrylamide gel and kappa-carrageenan gel. It wasfound that cells immobilized with kappa-carrageenan showed better thermal stability compared to those immobilized by polyacrylamide gel. The cells immobilized with kappa-carrageenan were treated with glutaraldehyde and hexamethylenediamine to prevent gel destruction during prolonged operation. By immobilizing intact cells, the optimal temperature for the synthetic enzyme reaction shifted higher by 8°C and the optimal pH became broader around 6.2 In continuous operation, the immobilized cells retained better operational stability at 25 than at 37°C, and also showed maximal conversion up to 83% at 25°C.
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Kim, I.H., Nam, D.H. & Ryu, D.D.Y. Cephalexin synthesis using immobilizedXanthomonas citri cells. Appl Biochem Biotechnol 8, 195–202 (1983). https://doi.org/10.1007/BF02778257
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DOI: https://doi.org/10.1007/BF02778257
Index Entries
- Cephalexin, synthesis using immobilized cells
- synthesis, of cephalexin using immobilized cells
- immobilized cells, in cephalexin synthesis
- Xanthomonas citri cells, cephalexin synthesis using immobilized
- polyacrylamide gel immobilization, ofXanthamonas citri cells
- k-carrageenan immobilization, ofXanthomonas citri