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Polyamide resin: A novel immunoadsorbent

I. Preparation of polyamide resin-antigen conjugates for use in a solid phase assay for radiolabeled antibody synthesized in vitro

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Abstract

A solid phase assay for radiolabeled antibody synthesizedde novo in vitro has been described (1). The solid phase consists of antigen covalently bound to bromacetyl cellulose, a useful but difficult to prepare immunoadsorbent. Herein, we describe the preparation of polyamide resin immunoadsorbent and the procedure for coupling antigen to the polymer. Data are presented that show that polyamide resin-Ag‡ conjugates can replace bromacetyl cellulose-Ag conjugates. The usefulness of this easily prepared and inexpensive immunoadsorbent is discussed.

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Abbreviations

BAC:

bromoacetyl cellulose

FIB:

bovine fibrinogen

HBSS:

Hanks' balanced salts solution

HRP:

horseradish peroxidase

IA:

immunoadsorbent

KLH:

keyhole limpet hemocyanin

MEM:

Eagle's minimal essential medium supplemented with non-essential amino acids, penicillin, streptomycin, HEPES buffer and normal rabbit serum

PAR:

polyamide resis

PBS:

phosphate-buffered saline

Reference

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Author information

Author notes

  1. Jonathan L. Kiel

    Present address: Brooks Air Force Base, San Antonio, Texas

Authors and Affiliations

  1. Department of Microbiology, Texas Tech University Health Sciences Center, School of Medicine, 79430, Texas, Lubbock, USA

    Gary T. Gooch & Sumner I. Schwarz

  2. Biochemistry, Texas Tech University Health Sciences Center, School of Medicine, Lubbock, 79430, Texas, USA

    Jonathan L. Kiel & Johannes Everse

Authors
  1. Gary T. Gooch
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  2. Sumner I. Schwarz
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  3. Jonathan L. Kiel
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  4. Johannes Everse
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Gooch, G.T., Schwarz, S.I., Kiel, J.L. et al. Polyamide resin: A novel immunoadsorbent. Appl Biochem Biotechnol 8, 105–114 (1983). https://doi.org/10.1007/BF02778091

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  • DOI: https://doi.org/10.1007/BF02778091

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