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Gastroenterologia Japonica

, Volume 6, Issue 4, pp 217–222 | Cite as

Characterization of the ester form bilirubin fraction showing positive phosphate ester reaction

  • Tadasuke Kondo
  • Toshio Kawai
  • Takehiko Yamamoto
  • Tetsuichi Izawa
Original Article
  • 10 Downloads

Summary

Positive phosphate-ester fraction of the ester-form bilirubin was prepared from the rat bile after intravenous injection of32P-sodium phosphate and also from the jaundiced urine of various liver diseases. The administered32P-radioactivity was mainly recovered in the third spot of ester-form bilirubin on the radioautogram and the radiochromatogram. The maxium absorption of the aqueous solution was 410-420, and that of its azo-pigment and acid azo-pigment was 550-560 mµ and 555-565 mµ, respectively. Photochemical and chemical properties were the same as we have previously reported on the ester-form bilirubin. Molar ratios of phosphate and bilirubin in this fraction were from 1.22 to 1.90 and ratios of Pigment A and Pigment B were from 0.10 to 0.41. These results suggest the existence of bilirubin diphosphate-ester.

Key words

direct bilirubin 32P-phosphate bilirubin phosphate azo-pigment radioautogram radioscintigram column chromatography paper chromatography 

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Copyright information

© The Japanese Society of Gastroenterology 1971

Authors and Affiliations

  • Tadasuke Kondo
    • 1
  • Toshio Kawai
    • 1
  • Takehiko Yamamoto
    • 1
  • Tetsuichi Izawa
    • 1
  1. 1.The First Department of Internal MedicineOkayama University Medical SchoolJapan

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