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Characterization of the ester form bilirubin fraction showing positive phosphate ester reaction

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Summary

Positive phosphate-ester fraction of the ester-form bilirubin was prepared from the rat bile after intravenous injection of32P-sodium phosphate and also from the jaundiced urine of various liver diseases. The administered32P-radioactivity was mainly recovered in the third spot of ester-form bilirubin on the radioautogram and the radiochromatogram. The maxium absorption of the aqueous solution was 410-420mµ, and that of its azo-pigment and acid azo-pigment was 550-560 mµ and 555-565 mµ, respectively. Photochemical and chemical properties were the same as we have previously reported on the ester-form bilirubin. Molar ratios of phosphate and bilirubin in this fraction were from 1.22 to 1.90 and ratios of Pigment A and Pigment B were from 0.10 to 0.41. These results suggest the existence of bilirubin diphosphate-ester.

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Authors and Affiliations

  1. The First Department of Internal Medicine, Okayama University Medical School, Japan

    Tadasuke Kondo, Toshio Kawai, Takehiko Yamamoto & Tetsuichi Izawa

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  1. Tadasuke Kondo
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  2. Toshio Kawai
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  3. Takehiko Yamamoto
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  4. Tetsuichi Izawa
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Kondo, T., Kawai, T., Yamamoto, T. et al. Characterization of the ester form bilirubin fraction showing positive phosphate ester reaction. Gastroenterol Jpn 6, 217–222 (1971). https://doi.org/10.1007/BF02777116

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  • DOI: https://doi.org/10.1007/BF02777116

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