HCV-RNA assay in peripheral blood mononuclear cells in relation to IFN therapy
- 10 Downloads
Hepatitis C virus RNA (HCV-RNA) was serially assayed in the serum and peripheral blood mononuclear cells (PBMC) of 10 patients with chronic hepatitis C who underwent interferon (IFN) therapy, and whether detection of HCV-RNA from PBMC serves as an index of the response of chronic hepatitis C to IFN therapy was evaluated. HCV-RNA was assayed by reversed transcription and polymerase chain reaction using the 5′-noncoding region as a primer. IFN therapy was effective in 3 patients and ineffective in the other 7 patients. HCV-RNA disappeared from the serum during and immediately after the IFN therapy in all 3 patients in whom the therapy was effective and in 3 of 7 patients in whom the therapy was ineffective. HCV-RNA desappeared from PBMC in all 3 patients in whom the therapy was effective, but PBMC HCV-RNA remained positive in 6 of the 7 patients in whom the therapy was ineffective, and the serum HCV-RNA became positive again in 5 of these 6 patients after 6 months. The disappearance of HCV-RNA from PBMC was associated with long-term stabilization of the serum alanine aminotransferase value, so HCV-RNA assay in PBMC is considered to be useful as a prognostic marker of chronic hepatitis C after IFN therapy.
Key wordsChronic hepatitis C PBMC IFN HCV-RNA
Unable to display preview. Download preview PDF.
- 11.Okamoto H, Okada S, Sugiyama Y, et al. Detection of hepatitis C virus RNA by a two-stage polymerase chain reaction with two pairs of primers deduced from the 5’noncoding region. Japan J Exp Med 1990;60:215–222.Google Scholar
- 15.Chayama K, Saitoh S, Arase Y, et al. Effect of interferon administration on serum hepatitis C virus RNA in patients with chronic hepatitis C. 1991;13:1040–1043.Google Scholar
- 17.Kanai K, Iwata K, Nakao K, et al. Suppression of hepatitis C virus RNA by interferon-α. 1990;336:245.Google Scholar
- 22.Nakatsuji Y, Matsumoto A, Tanaka E, et al. Detection of chronic hepatitis C virus infection by four diagnostic systems: First-generation and second-generation enzyme-linked immunosorbent assay, second-generation recombinant immunoblot assay and nested polymerase chain reaction analysis. Hepatology 1992;16:300–305.PubMedCrossRefGoogle Scholar
- 24.Kondo T. Evaluation of the antiviral effect of interferon therapy for chronic hepatitis C. Acta Hepat Jap 1992;33:591–599. (in Japanese with English abstracts)Google Scholar
- 27.Kato N, Yokosuka O, Omata M, et al. The relationship between the amount of hepatitis C virus quantified by competitive RT-PCR (CRT-PCR) and the effect of interferon treatment. Acta Hepat Jap 1991;32:750–751. (in Japanese)Google Scholar