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Gastroenterologia Japonica

, Volume 22, Issue 6, pp 743–747 | Cite as

The protective effect of cyclosporin a on experimentally-induced acute hepatic injury in mice

  • Yasuhiro Mizoguchi
  • Hiroshi Kuboi
  • Chie Kodama
  • Yoshihide Sakagami
  • Shuichi Seki
  • Kenzo Kobayashi
  • Sukeo Yamamoto
  • Seiji Morisawa
Original Article

Summary

When heat-killedPropionibacterium acnes (P. acnes) and a small amount of endotoxin lipopolysaccharide (LPS) were intravenously injected into mice at a week’s interval, most of them died of massive hepatic cell necrosis. This experimentally-induced acute liver injury was significantly inhibited by cyclosporin A (CsA), resulting in a remarkable improvement of the survival rate. This protective effect of CsA on acute liver injury was also histopathologically confirmed. To study the mechanism by which CsA protected the mice from fatal hepatic injury, adherent cells prepared from the murine liver 7 days afterP. acnes injection were incubated with LPS in the presence of CsA, and the effect of CsA on the production of the cytotoxic factor from the adherent cells was estimated. As a result, CsA inhibited the activation of liver adherent cells and suppressed the release of the cytotoxic factor.

Key words

Acute hepatic injury Cyclosporin A Cytotoxic factor Lipopolysaccharide Propionibacterium acnes 

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References

  1. 1).
    Tsutsui H, Mizoguchi Y, Yamamoto S, et al: Studies on experimentally-induced acute hepatic failure: Possible involvement of activated liver adherent cells, in “Cells of the hepatic sinusoid” vol. 1 by Kirn A, et al, The Kupffer Cell Foundation, Netherlands. 1986; 307–314Google Scholar
  2. 2).
    Tsutsui H, Miyajima K, Mizoguchi Y, et al: Studies on experimentally-induced acute hepatic failure in mice. Characteristics of the hepatocytotoxic factors released from the activated liver adherent cells. Jpn J Gastroenterol 1986; 83: 1161–1167 (Jpn)Google Scholar
  3. 3).
    Mochizuki M: Effects of cyclosporin on the efferent limb of the immune response. Transplantation 1983; 35: 148Google Scholar
  4. 4).
    Bunjes D, Hatdt C, Solbach W, et al: Studies on the mechanism of action of Cyclosporin A in the murine and human T-cell response in vitro, in “Cyclosporin A” by White DJG, Elsevier Biomedical Press, Amsterdam. 1982; 261–280Google Scholar
  5. 5).
    Drath DB, Kahan BD: Alterations in rat pulmonary macrophages function by the immunosuppressive agents cyclosporin, azathioprine and prednisolone. Transplantation 1983; 35: 588–592PubMedCrossRefGoogle Scholar
  6. 6).
    Bellemann P, Gebhardt R, Mecke D, et al: An improved method for the isolation of hepatocytes from liver slices. Anal Biochem 1977; 81: 408–415PubMedCrossRefGoogle Scholar
  7. 7).
    Ferluga J, Allison AC: Role of mononuclear infiltrating cells in pathogenesis of hepatitis. Lancet 1978; II:610–611CrossRefGoogle Scholar

Copyright information

© The Japanese Society of Gastroenterology 1987

Authors and Affiliations

  • Yasuhiro Mizoguchi
    • 1
  • Hiroshi Kuboi
    • 1
  • Chie Kodama
    • 1
  • Yoshihide Sakagami
    • 1
  • Shuichi Seki
    • 1
  • Kenzo Kobayashi
    • 1
  • Sukeo Yamamoto
    • 2
  • Seiji Morisawa
    • 3
  1. 1.Third Department of Internal MedicineOsaka City University Medical SchoolOsakaJapan
  2. 2.Osaka Socio-Medical Center HospitalOsakaJapan
  3. 3.First Department of BiochemistryOsaka City University Medical SchoolOsakaJapan

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