Studies on effect of carcinoembryonic antigen on leucocyte migration inhibition test in patients with colorectal cancer
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Leucocyte migration inhibition test (LMIT) is a useful method to detect tumor associated antigens (TAA) in cancer patients. Carcinoembryonic antigen (CEA) is one of the best tumor markers for gastrointestinal cancer, and especially for colorectal cancer patients, who show high plasma CEA level frequently. In this study, we performedLMIT in 71 colorectal cancer patients with 3 M KC1 extracts of cancer tissues and measured concentration of CEA in the plasma and the extracts of cancer tissue simultaneously. Although CEA in colorectal cancer extracts was individually varied from low to high in concentration, the levels of CEA had no relation toLMI reactivity. In addition, theLMI reactivity of colorectal cancer patients did not relate to the plasmaCEA level of the corresponding patient.
The results suggest that some antigens which induce leucocyte migration inhibition factor (LMIF) to lymphocytes from colorectal cancer patients might be different substances from CEA.
Key Wordsleucocyte migration inhibition test (LMIT) arcinoembryonic antigen (CEA) colorectal cancer
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- 1).Clausen JE: Tuberculin-induced migration inhibition of human peripheral leucocytes in agarose medium. J Acta Allerg (Kbh) 26: 56–80, 1971Google Scholar
- 3).Kojima O, Fujita Y, Iwai N, Yamane T, Kitagawa N, Nishioka B, Majima S: Studies on cell-mediated immunity in patients with colorectal cancer: Leucocyte migration inhibition test using allogeneic 3 M KC1 extracts. Aus NZ Surg 51: 434–439, 1981Google Scholar
- 4).Lowry OH, Rosenbrough NJ, Farr AL, Randall RJ: Protein measurement with the folin phenol reagent. Biol Chem 193: 265–275, 1951Google Scholar
- 7).Ritts RE, Shani A, Weiland LH, Go VLW, Leduc P: Leucocyte adherence inhibition, carcinoembryonic antigen, and tumor size in patients with colorectal carcinoma. In “Assessment of immune status by the leucocyte adherence inhibition test” ed DMP Thomson, pp 173–184, 1982, Academic press, New YorkGoogle Scholar