Diphenylamine reaction of gastric juice correlation of the reaction with histochemical and histoenzymological findings of gastric mucosa
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DPA reaction of gastric juice was carried out on 300 subjects consisting of 289 patients with various diseases of the gastrointestinal tract and 11 healthy volunteers. In 80 of these subjects, histochemical and histoenzymological examinations were carried out on biopsy specimens of gastric mucosa.
In patients with gastric carcinoma or with diseases which could be considered as being precancerous, the extinction values of DPA reaction of gastric juice were significantly higher than the values in healthy controls and in patients with definitely benign conditions.
The enhanced extinction values of DPA reaction were usually accompanied by evidences of atrophic gastritis with intestinal metaplasia, and increased acid mucopolysaccharide contents as well as enhanced enzymic activities of alkaline and acid phosphatases, non-specific E-600 resistant esterase and of lactic dehydrogenase were observed.
In 172 patients, follow-up studies for not less than 2 years were available, and 214 in 2 of these, originallylow extinction values were elevated during the observation period, and the elevation in each case was found to be accompanied by malignant transformation of gastric mucosa. Ingestions of acylpyrin, prednison and carbohydrate-containing diet, as well as excessive abuse of ethanol, were found to affect the results of DPA reaction, furnishing another support to the sensitive reflection of minor changes in gastric mucosa upon DPA reaction. Thus DPA reaction of gastric juice was found to be of value in the diagnosis and follow-up observations of gastric diseases, if applied in combination with other routine methods of examination.
KeywordsGastric Juice Gastric Mucosa Gastric Carcinoma Intestinal Metaplasia Functional Dyspepsia
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