Gastroenterologia Japonica

, Volume 27, Issue 1, pp 54–60 | Cite as

Changes in the markers related to collagen synthesis in the liver of chronically alcohol treated rats

  • Shujiro Takase
  • Akira Takada
  • Sachio Urashima
  • Keiko Enyama
  • Mari Fujii
Liver, Pancreas, and Biliary Tract


In order to clarify the roles of Ito cells in the development of alcoholic fibrosis, markers related to collagen synthesis in the liver were analyzed in chronically alcohol treated rats. The livers were obtained from rats fed a diet containing alcohol (alcohol group) and those fed a control diet (control group) for 4 weeks. Prolyl hydroxylase (PH) activity in the whole liver tissue did not differ in the alcohol and control groups. However, the activity in the isolated Ito cells was significantly higher in the alcohol group than in the control group. Immunoreactive PH beta-subunit contents in the liver and serum were significantly higher in the alcohol group than in the control group. Hydroxyproline contents in the livers did not differ in either groups. Immunohistochemically, type IV collagen and laminin were clearly stained along with the sinusoid in the livers of the alcohol group. However, the staining reactions were very weak in the control group. Staining reactions to types I and III collagen were very weak or almost absent in the livers of both groups. Desmin-positive cells, along with the sinusoid, increased significantly in the alchol group, especially at the centrilobular area, suggesting that the number of Ito cell increase in the centrilobular areas of the alcohol treated rats. These results suggest that type IV collagen and laminin synthesis increase in the Ito cells of chronically alcohol treated rats, although clear evidence of hepatic fibrosis was not obtained. This increase may be related to capillarization of the sinusoids and finally to the development of perisinusoidal fibrosis in alcoholics.

Key words

chronic alcoholic rats collagen synthesis Ito cells laminin type IV collagen 


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Copyright information

© The Japanese Society of Gastroenterology 1992

Authors and Affiliations

  • Shujiro Takase
    • 1
  • Akira Takada
    • 1
  • Sachio Urashima
    • 1
  • Keiko Enyama
    • 1
  • Mari Fujii
    • 1
  1. 1.Division of Gastroenterology, Department of Internal MedicineKanazawa Medical UniversityUchinada, IshikawaJapan

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