Summary
To evaluate the clinical applications of serum thymidine kinase (TK) activity, we compared the results obtained with this parameter with those of other liver function tests in 27 patients with acute viral hepatitis and 16 normal controls. In those in the acute stage, the serum TK activity increased significantly to 55.5 ± 66.5 U/L. There was no significant correlation between serum TK activity and findings for serum albumin, bilirubin, alkaline phosphatase or r-glutamyle transpeptidase. However, it did correlate significantly well with the serum activity of aspartate aminotransferase (AST) (r=0.621, P<0.01), alanine aminotransferase (ALT) (r=0.551, P<0.01), and lactate dehydrogenase (LDH) (r=0.620, P<0.01). Serum TK activity reached higher than 70 U/L in 8 of 11 patients with hepatitis A; however, no patients with the other types of hepatitis reached such a high level. During the recovery stage, the serum TK activity decreased significantly to 5.9± 1.7 U/L (P<0.01), and did not correlate with AST, ALT, LDH or other conventional liver function parameters. The data suggest that an elevation of serum TK in patients with acute viral hepatitis results from hepatocellular damage. A marked elevation of serum TK activity may thus provide a marker for acute hepatitis A infection.
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References
Bollum FJ, Van Potter R. Nucleic acid metabolism in regenerating rat liver. (6). Soluble enzymes which convert thymidine to thymidine phosphates and DNA. Cancer Res 1959; 19:561–565.
Klemper HG, Haynes GR. Thymidine kinase in rat liver during development. Biochem J 1968;108:541–546.
Bresnick E, Mayfield ED Jr, Liebelt AG, et al. Enzyme patterns in, a group of transplantable mouse hepatomas of different growth rates. Cancer Res 1971;31:743–751.
Kit S. Viral-associated and induced enzymes. Pharmacol Therapy 1979;4:501–585.
Ellims PH, Gan TE, Medley G, et al. Prognostic relevance of thymidine kinase isozymes in adult non-Hodgkin’s lymphoma. Blood 1981;58:926–930.
Kreis W, Arlin Z, Yagoda A, et al. Deoxycytidine and deoxythymidine kinase activities in plasma of mice and patients with neoplastic disease. Cancer Res 1982;42:2514–2517.
Taylor A, Hewitt EG Jr, Jones OW. Tumor-associated thymidine kinase in the sera of rats with transplanted hepatomas. Cancer Res 1976;36:2070–2072.
Gronowitz JS, Kallander CFR, Diderholm H, et al. Application of an in vitro assay for serum thymidine kinase: results on viral disease and malinancies in humans. Int J Cancer 1984;33: 5–12.
Kallander CFR, Simonsson B, Hagberg H, et al. Serum deoxythymidine kinase gives prognostic information in chronic lymphocytic leukemia. Cancer 1984;54:2450–2455.
Hagberg H, Glimelius B, Gronowitz S, et al. Biochemical markers in non-Hodgkin’s lymphoma stage III and IV and prognosis: a multivariate analysis. Scand J Haematol 1984;33:59–67.
Bello LJ. Regulation of thymidine kinase synthesis in human cells. Exp Cell Res 1974;89:263–274.
Frederiks WM, Myagkaya GL, Bosch KS, et al. The value of enzyme leakage for the prediction of necrosis in liver ischemia. Histochemistry 1983;78:459–472.
Tanaka K, Ohkawa S, Nishino T, et al. Role of the hepatic branch of the vagus nerve in liver regeneration in rats. Am J Physiol 1987;253:G439-G444.
Littlefield JW. The periodic synthesis of thymidine kinase in mouse fibroblasts. Biochem Biophys Acta 1966; 114:398–403.
Adler R, McAuslan BR. Expression of thymidine kinase variants is a function of the replicative state of cells. Cell 1974;2:113–117.
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Tanaka, K., Sishido, T., Morimoto, M. et al. Elevated serum thymidine kinase activity in patients with acute viral hepatitis. Gastroenterol Jpn 28, 51–55 (1993). https://doi.org/10.1007/BF02775003
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DOI: https://doi.org/10.1007/BF02775003