Immunohistochemical detection of biotinlabeled murine monoclonal antibody in nude mice transplanted with human colon cancer
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The tissue localization of biotin-labeled murine monoclonal antibody (MAb) A7 directed against human colonic cancer was examined in tumor-bearing nude mice following intravenous administration. MAb A7 did not react with any murine visceral organs. At 24 hr after biotin-labeled A7 injection, the tumor was stained by the avidin-biotin peroxidase complex method, but no staining was observed after injection of biotin-labeled normal mouse Ig G in the control group. Biotin-labeled MAb. was found to be suitable for the detection of the xenografted tumor of nude mice. Our purpose was to provide a new method concerning the distribution of biotin-labeled MAbin vivo.
Key wordsA7 biotin-labeled antibody Immunohistochemistry Monoclonal antibody Nude mouse
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