Summary
The tissue localization of biotin-labeled murine monoclonal antibody (MAb) A7 directed against human colonic cancer was examined in tumor-bearing nude mice following intravenous administration. MAb A7 did not react with any murine visceral organs. At 24 hr after biotin-labeled A7 injection, the tumor was stained by the avidin-biotin peroxidase complex method, but no staining was observed after injection of biotin-labeled normal mouse Ig G in the control group. Biotin-labeled MAb. was found to be suitable for the detection of the xenografted tumor of nude mice. Our purpose was to provide a new method concerning the distribution of biotin-labeled MAbin vivo.
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This study was supported in part by a Grant-in-Aid for Cancer Research from the Ministry of Education, Science and Culture, Japan, and by a grant from the Ministry of Health and Welfare, Japan.
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Yokota, T., Takahashi, T., Yamaguchi, T. et al. Immunohistochemical detection of biotinlabeled murine monoclonal antibody in nude mice transplanted with human colon cancer. Gastroenterol Jpn 24, 22–24 (1989). https://doi.org/10.1007/BF02774866
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DOI: https://doi.org/10.1007/BF02774866