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Gastroenterologia Japonica

, Volume 24, Issue 1, pp 22–24 | Cite as

Immunohistochemical detection of biotinlabeled murine monoclonal antibody in nude mice transplanted with human colon cancer

  • Takashi Yokota
  • Toshio Takahashi
  • Toshiharu Yamaguchi
  • Kazuya Kitamura
  • Kiyoshi Sawai
Original Article
  • 15 Downloads

Summary

The tissue localization of biotin-labeled murine monoclonal antibody (MAb) A7 directed against human colonic cancer was examined in tumor-bearing nude mice following intravenous administration. MAb A7 did not react with any murine visceral organs. At 24 hr after biotin-labeled A7 injection, the tumor was stained by the avidin-biotin peroxidase complex method, but no staining was observed after injection of biotin-labeled normal mouse Ig G in the control group. Biotin-labeled MAb. was found to be suitable for the detection of the xenografted tumor of nude mice. Our purpose was to provide a new method concerning the distribution of biotin-labeled MAbin vivo.

Key words

A7 biotin-labeled antibody Immunohistochemistry Monoclonal antibody Nude mouse 

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Copyright information

© The Japanese Society of Gastroenterology 1989

Authors and Affiliations

  • Takashi Yokota
    • 1
  • Toshio Takahashi
    • 1
  • Toshiharu Yamaguchi
    • 1
  • Kazuya Kitamura
    • 1
  • Kiyoshi Sawai
    • 1
  1. 1.The First Department of SurgeryKyoto Prefectural University of MedicineKyotoJapan

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