Summary
The culture supernatant of activated lymphocytes was shown to contain macrophage activating factor (MAF), a kind of lymphokine, which activated the peritoneal macrophages prepared from guinea pigs. When the culture fluid of the MAF-activated macrophages was added to the isolated liver cells, a significant inhibition of their albumin biosynthesis was demonstrated. The active material was recovered in a definitive fraction by gel filtration using a Sephadex G-75 column and this was further fractionated into two fractions by DEAE-cellulose column chromatography, suggesting that at least two kinds of active substances existed. By isoelectric focusing electrophoresis it was shown that these two active principles were substances having different isoelectric points, by isoelectric focusing electrophoresis.
These results suggest that some cytotoxic or cytostatic materials are produced from the lymphokine-activated macrophages and they may participate in the occurence of liver injury at least partially.
Similar content being viewed by others
References
Meyer zum Büschenfelde KH, et al: Experimental chronic active hepatitis in rabbits following immunization with human liver proteins. Clin Exp Immunol 11: 99, 1972
Miller J, et al: Cell-mediated immunity to a human liver specific antigen in patients with active chronic hepatitis and primary billiary cirrhosis. Lancet II: 296, 1972
Hopf U, et al: Liver specific antigens of different species. II Localization of a membrane antigen at the cell surface of isolated hepatocytes. Clin Exp Immunol 16: 117, 1974
Meyer zum Büschenfelde KH, Hopf U: Studies on the pathogenesis of experimental chronic active hepatitis in rabbits. Induction of the disease and protective effect of allogeneic liver specific protein. Br J Exp Pathol 55: 498, 1974
Mizoguchi Y, et al: Immunological studies on chronic active hepatitis: Possible involvement of macrophage mediated cytotoxicity in its immunopathogenesis. Hepato-gastroenterol 28: 250, 1981
Mizoguchi Y, et al: Possible involvement of macrophage-mediated hepatocytotoxicity in chronic active hepatitis: The induction of liver cell injury by culture supernatant of MAF-activated macrophages. Hepatogastroenterol 28: 254, 1981
Page RC: The macrophages as a secretory cell. Int Rev Cytol 52: 119, 1978
Meyer zum Büschenfelde KH, Miescher PA: Liver specific antigen — Purification and characterization. Clin Exp Immunol 10: 89, 1972
Bellemann P, et al: An improved method for the isolated of hepatocytes from liver slice. Selective removed of trypanblue dyeable cells. Analytical Biochem 81: 408, 1977
Ferluga J, Allison AC: Role of mononuclear infiltrating cells in pathogenesis of hepatitis. Lancet I: 610, 1978
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Mizoguchi, Y., Tsutsui, H., Monna, T. et al. Studies on the mechanism of liver injury by macrophage-mediated cytotoxicity—partial purification of cytotoxic factor detected in the culture supernatant of activated macrophages. Gastroenterol Jpn 18, 41–46 (1983). https://doi.org/10.1007/BF02774858
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02774858