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Gastroenterologia Japonica

, Volume 18, Issue 1, pp 41–46 | Cite as

Studies on the mechanism of liver injury by macrophage-mediated cytotoxicity—partial purification of cytotoxic factor detected in the culture supernatant of activated macrophages

  • Yasuhiro Mizoguchi
  • Hiroko Tsutsui
  • Takeyuki Monna
  • Sukeo Yamamoto
  • Seiji Morisawa
Original Article

Summary

The culture supernatant of activated lymphocytes was shown to contain macrophage activating factor (MAF), a kind of lymphokine, which activated the peritoneal macrophages prepared from guinea pigs. When the culture fluid of the MAF-activated macrophages was added to the isolated liver cells, a significant inhibition of their albumin biosynthesis was demonstrated. The active material was recovered in a definitive fraction by gel filtration using a Sephadex G-75 column and this was further fractionated into two fractions by DEAE-cellulose column chromatography, suggesting that at least two kinds of active substances existed. By isoelectric focusing electrophoresis it was shown that these two active principles were substances having different isoelectric points, by isoelectric focusing electrophoresis.

These results suggest that some cytotoxic or cytostatic materials are produced from the lymphokine-activated macrophages and they may participate in the occurence of liver injury at least partially.

Key Words

macrophage mediated cytotoxicity activated macrophage cytotoxic factor monokine 

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Copyright information

© The Japanese Society of Gastroenterology 1983

Authors and Affiliations

  • Yasuhiro Mizoguchi
    • 1
    • 2
  • Hiroko Tsutsui
    • 1
    • 2
  • Takeyuki Monna
    • 1
    • 2
  • Sukeo Yamamoto
    • 1
    • 2
  • Seiji Morisawa
    • 1
    • 2
  1. 1.The Third Department of Internal MedicineOsaka City University Medical SchoolOsakaJapan
  2. 2.The First Department of BiochemistryOsaka City University Medical SchoolOsakaJapan

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