Advertisement

Gastroenterologia Japonica

, Volume 10, Issue 2, pp 141–145 | Cite as

Studies of ultramicrostructure in relation to disaccharidase activity in the jejunal mucosa

  • Yutaka Yoshida
  • Giro Sasaki
  • Shohei Goto
  • Shigetoshi Yanagiya
  • Norio Nakasato
  • Kazuo Takashina
  • Aiichiro Ito
  • Fujio Matsunaga
Article
  • 6 Downloads

Summary

Ultramicrostructural changes of the mucosa of the small intestine were studied in relation to the alterations of the lactase activity, measuring the height of microvilli by electron microscopy and the enzyme activity by the method of Dahlqvist.

Studies with clinical cases revealed that the height of microvilli correlated with the lactase activity significantly (p<0.01), and that patients with relatively higher microvilli had a correspondingly higher activity of lactase. In animal experiments an increase of lactase activity by lactose load feeding and low calorie feeding resulted in a raise in the height of microvilli.

It was concluded that lactase deficiency in adults and in various gastrointestinal diseases, where no histological abnormality was observed by light microscopy is perhaps due to shortening of the microvilli of the mucosa. It was speculated that the microvillous change and the sequential hypolactasia may be the initial ultramicrostructural and enzymatic alteration in the diffuse disease of the small intestine.

Key Words

Disaccharidase Lactase Microvilli Jejunal mucosa Ultramicrostructure 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1).
    Sheehy, T.W. and Anderson, P.R.: Disaccharidase activity in normal and diseased small bowel. Lancet, 2: 1–4, 1965.CrossRefGoogle Scholar
  2. 2).
    Dahlqvist, A. et al.: Intestinal lactase deficiency and lactose intolerance in adults. Gastroenterol., 45: 488–491, 1963.Google Scholar
  3. 3).
    Haemmerli, U.P. et al.: Lactasemangel der Dunndarmmucosa als Ursache gewisser Formen erworbener Milchintoleranz beim Erwachsenen. Hael. Med. Acta., 30: 693–705, 1963.Google Scholar
  4. 4).
    Newcomer, A.D. and McGill, D.B.: Distribution of disaccharidase activity in the small bowel of normal and lactase-deficient subjects. Gastroenterol., 51: 481–488, 1966.Google Scholar
  5. 5).
    Holzel, A.: Development of intestinal enzyme systems, and its relation to diarrhea. Ped. Clin. North Amer., 12: 635–654, 1965.Google Scholar
  6. 6).
    Townley, R.R.W.: Disaccharidase deficiency in infancy and childhood. Pediat., 38: 127–141, 1966.Google Scholar
  7. 7).
    Plotkin, G.R. and Isselbacher, K.J.: Secondary disaccharidase deficiency in adult celiac disease (Non-tropical sprue) and other malabsorption states. New Eng. J. Med., 271: 1033–1037, 1964.PubMedCrossRefGoogle Scholar
  8. 8).
    Yoshida, Y.: Biopsy and disaccharidase activity of the small-bowel mucosa. Jap. J. Gastroenterology, 66: 499–500, 1969 (Japanese).Google Scholar
  9. 9).
    Sasaki, G.: Intestinal lactase activity in Japanese adults. Jap. J. Gastroenterology, 68: 37–49, 1971 (Japanese).Google Scholar
  10. 10).
    Brandborg, L.L. et al.: A multipurpose instrument for suction biopsy of the esophagus, stomach, small bowel, and colon. Gastroenterology, 37: 1–16, 1959.PubMedGoogle Scholar
  11. 11).
    Dahlquist, A.: Method for assay of intestinal disaccharidases. Anal. Biochem., 7: 18–25, 1964.CrossRefGoogle Scholar
  12. 12).
    Lowry, O.H. et al.: Protein measurement with the folin phenol reagent. J. Biol. Chem., 193: 265–275, 1951.PubMedGoogle Scholar
  13. 13).
    Brown, A.L.: Microvilli of the human jejunal epithelial cell. J. Cell Biol., 12: 623–627, 1962.PubMedCrossRefGoogle Scholar
  14. 14).
    Yoshida, Y. et al.: Disaccharidase activity in adults, with emphasis on the diagnosis and therapy of the deficient-states. Jap. J. of Clin, and Exp. Med., 47: 1156–1163, 1970 (Japanese).Google Scholar
  15. 15).
    Goto, S.: Disaccharidase activity in the jejunal mucosa. Jap. J. Int. Med., 62: 747–755, 1963 (Japanese).Google Scholar
  16. 16).
    Yoshida, Y. et al.: Studies on intestinal sugar absorption. Jap. J. Gastroenterology, 70: 1286, 1973 (Japanese).Google Scholar

Copyright information

© The Japanese Society of Gastroenterology 1975

Authors and Affiliations

  • Yutaka Yoshida
    • 1
  • Giro Sasaki
    • 1
  • Shohei Goto
    • 1
  • Shigetoshi Yanagiya
    • 1
  • Norio Nakasato
    • 1
  • Kazuo Takashina
    • 1
  • Aiichiro Ito
    • 1
  • Fujio Matsunaga
    • 1
  1. 1.1st Department of Internal MedicineHirosaki University School of MedicineHirosakiJapan

Personalised recommendations