Summary
Antibody-dependent cellular cytotoxicity (ADCC) of peripheral blood lymphocytes for Chang cells coated with antibody to liver specific membrane lipoprotein was studied in patients with chronic active liver diseases (CALD) to investigate the pathogenesis of liver cell injury.
Of 20 CALD patients, a reduced ADCC was found in 5 cases, whereas an increased ADCC was noted in 8 cases. The remaining 7 cases showed a normal ADCC. There was a significant correlation (p<0.01) between a decreased ADCC and an increased level of immune complexes in the sera of CALD patients when a125I-Clq binding test was used.
When sera from ADCC patients, which had a high level of circulating immune complexes, or aggregated human IgG was added, ADCC of normal lymphocytes was significantly inhibited (p<0.01) compared with that of lymphocytes alone.
These results suggest that circulating immune complexes play a role on the pathogenesis of liver damage in CALD.
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References
Paronetto F, et al: Immunological studies in patients with chronic active hepatitis: cytotoxic activity of lymphocytes to autochthonous liver cells grown in tissue culture. Clin Exp Immunol 19: 99, 1975
Wands JR, et al: Lymphocyte cytotoxicity to autologous cells in chronic active hepatitis. Proc Natl Acad Sci USA 72: 1301, 1975
Geubel AP, et al: Lymphocyte cytotoxicity and inhibition studied with autologous liver cells: observations in chronic active liver disease and the primary biliary cirrhosis syndrome. Gastroenterology 74: 450, 1976
Thomson AD, et al: Lymphocyte cytotoxicity to isolated hepatocytes in chronic active hepatitis. Nature (Lond)252: 721, 1974
Cochrane AMG, et al: Antibody-dependent cell-mediated (K cell) cytotoxicity against isolated hepatocytes in chronic active hepatitis. Lancet 1: 441, 1976
Wands JR, et al: Cell-mediated immunity in acute and chronic hepatitis. J Clin Invest 55: 921, 1975
Kakumu S, et al: Lymphocyte cytotoxicity against Chang liver cells in chronic active hepatitis. Cell Immunol 36: 46, 1978
Kawanishi H: In vitro studies on IgG-mediated lymphocyte cytotoxicity in chronic active liver disease. Gastroenterology 73: 549, 1977
Bortolotti F, et al: Antibody dependent cellular cytotoxicity (ADCC) in acute hepatitis B and in chronic active hepatitis. Clin Exp Immunol 33: 211, 1978
Kawanishi H, et al: K-cell-mediated antibody-dependent cellular cytotoxicity in chronic active liver disease. Gastroenterology 76: 151, 1979
Kakumu S, et al: Inhibition of lymphocyte cytotoxicity in chronic active hepatitis. Clin Exp Immunol 33: 71, 1978
Chalmers TC, et al: Chronic active hepatitis, in “Diseases of the liver and biliary tract: standardization of nomenclature, diagnostic criteria and diagnostic methodology” by Leevy CM, Popper H, Sherlock S. Castle House publications Ltd. London, 1979, p 10
Kakumu S, et al: Occurrence and significance of antibody to liver-specific membrane lipoprotein by double-antibody immunoprecipitation method in sera of patients with acute and chronic liver diseases. Gastroenterology 76: 665, 1979
McFarlane IG, et al: Purification and characterization of human liver-specific membrane lipoprotein (LSP). Clin Exp Immunol 27: 381, 1977
Zubler RH, et al: Detection of immune complexes in unheated sera by a modified125I-Clq binding test. Effect of heating on the binding of Clq by immune complexes and application of the test to systemic lupus erythematosus. J Immunol 116: 232, 1976
Yonemasu K, et al: Rapid purification method for preparation of monospecific antisera and for biochemical studies. J Immunol 106: 304, 1971
Heusser C, et al: Effect of chemical and enzymatic radioiodination on in vitro human Clq activities. J Immunol 110: 820, 1973
Fye KH, et al: Immune complexes in hepatitis B antigen-associated periateritis nodosa. Detection by antibody-dependent cell-mediated cytotoxicity and the Raji cell assay. AmJ Med 62: 783, 1977
Macsween RNM, et al: Peripheral blood K cells and antibody-dependent lymphocyte cytotoxicity in chronic active hepatitis and primary biliary cirrhosis. (Abstr) Digestion 14: 532, 1976
Thomas HC, et al: Peripheral blood lymphocyte populations in chronic liver disease. Clin Exp Immunol 26: 222, 1976
Eckharat R, et al: K-lymphocytes (killer-cells) in Crohn’s disease and acute virus B-hepatitis. Gut 18: 1010, 1977
Morimoto T: Killer cell (k-cell) population in chronic liver disease. Acta Hepato Jpn 28: 985, 1980
Allison AC, et al: Cooperating and controlling functions of thymus-derived lymphocytes in relation to autoimmunity. Lancet 2: 135, 1971
Dudley FJ, et al: Cellular immunity and hepatitis-associated, Australia antigen liver disease. Lancet 1: 723, 1972
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Kakumu, S., Hotta, R. & Kuriki, J. Antibody dependent cellular cytotoxicity in chronic active liver diseases. Gastroenterol Jpn 16, 372–376 (1981). https://doi.org/10.1007/BF02774470
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DOI: https://doi.org/10.1007/BF02774470