Gastroenterologia Japonica

, Volume 22, Issue 3, pp 292–302 | Cite as

A study on the cell kinetics of the canine gastric mucosa by the cytofluorometric method: An evaluation of chemically induced gastric cancer

  • Yuji Amano
  • Shiro Fukumoto
Original Article


The cell kinetic alteration in the background mucosa of canine gastric cancer induced by N-ethyl-N′-nitro-N-nitrosoguanidine (ENNG) was evaluated by cytofluorometry in which the rate of S and G2 + M phase cell in gastric mucosal cells could be calculated, and a triphasic alteration was demonstrated; an initial reduction phase, an increase phase and a plateau phase with a high value. The initial reduction phase was caused by non-specific toxity of ENNG as observed in drug induced gastric mucosal lesions, and subsequent increase and plateau phases originated from the action of ENNG itself to activate the mucosal turn-over and from histological changes in the background mucosa such as regenerative hyperplastic change after mucosal erosion and atrophic changes, sometimes including intestinal metaplastic change. Further, in comparison to carcinogenesis in chemically induced gastric cancer with and without a surfactant (Tween 60), it was suggested that one of the promotion effects of Tween 60 was closely related with activation of the mucosal turn-over.

Key Words

Appearance rate of over 3C cells Cell kinetics Cytofluorometry ENNG induced canine gastric cancer 


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Copyright information

© The Japanese Society of Gastroenterology 1987

Authors and Affiliations

  • Yuji Amano
    • 1
  • Shiro Fukumoto
    • 1
  1. 1.Department of Internal Medicine (Second Unit)Shimane Medical UniversityShimane-kenJapan

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