Gastroenterologia Japonica

, Volume 22, Issue 2, pp 158–165 | Cite as

Human T cell clones reactive with hepatitis B virus (HBV) surface antigen from a HBV vaccine recipient

  • Akitaka Nonomura
  • Goroku Ohta
Original Article


Five T-cell clones reactive with HBsAg were obtained from peripheral blood lymphocytes of a HBV vaccine recipient by means of in vitro stimulation of lymphocytes for 5 days by HBsAg and subsequent re-culture of lymphocytes with HBsAg, feeder cells and T cell growth factor (TCGF) for 6 days, followed by the limiting dilution method. Clones, and subclones derived from one original clone showed a specific proliferative response to HBsAg in the presence of autologous feeder cells but not to unrelated antigens, such as influenza virus antigens, herpes simplex antigens, toxoplasma antigens, and PPD. All clones were found to have a surface marker of helper/inducer phenotype, Leu 1+, Leu 2a-, and Leu 3a+, detected by an indirect immunofluorescence method. These clones, however, did not show a substantial helper effect for in vitro anti-HBs production by autologous B cells. This suggests that the mechanism of the helper effect by specific T-cell clones is implicated, and functions of such clones other than specific helper effect on antibody-producing B cells must be considered.

Key Words

Feeder cells Hepatitic B virus surface antigen (HBsAg) T cell clones T cell growth factor (TCGF) 


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  1. 1).
    Dudley FJ, et al: Cellular immunity and hepatitis-associated Australia antigen disease. Lancet 1972, 1: 723PubMedCrossRefGoogle Scholar
  2. 2).
    Eddieston ALWF, et al: Inadequate antibody response to HBs antigen or suppressor T-cell defect in development of active chronic hepatitis. Lancet 1974, 2: 1543CrossRefGoogle Scholar
  3. 3).
    Thomas HC, et al: Immunological mechanisms in chronic hepatitis B infection. Hepatology 1982, 2: 116sGoogle Scholar
  4. 4).
    Paronetto F: The immunoregulatory network in liver disease: an overview. Liver 1982, 2: 255Google Scholar
  5. 5).
    Nonomura A, et al: Disordered immunoregulatory functions in patients with chronic active hepatitis. Clin Exp Immunol 1982, 47: 595PubMedGoogle Scholar
  6. 6).
    Thomas HC, et al: Inducer and suppressor T-cells in hepatitis B virus infection. Hepatology 1982, 2: 202PubMedCrossRefGoogle Scholar
  7. 7).
    Morgan DA, et al: Selective in vitro growth of T lymphocytes from normal bone marrows. Science 1976, 193: 1007PubMedCrossRefGoogle Scholar
  8. 8).
    Malissen B, et al: Clones of human cytotoxic T lymphocytes derived from an allosensitized individual. HLA specificity and cell surface markers. Scand J Immunol 1981, 14: 213PubMedCrossRefGoogle Scholar
  9. 9).
    Bach FH, et al: Cloning of T lymphocytes in man, in “Isolation, Characterization, and Utilization of T lymphocyte Clones”, by Fathman CG and Fitch FW. Academic Press, New York, 1982: 413Google Scholar
  10. 10).
    Fathman CG, et al: Clones of alloreactive T cells. Nature 1978, 272: 617PubMedCrossRefGoogle Scholar
  11. 11).
    Kimoto M, et al: Antigen-reactive T cell clones. I. Transcomplementing hybrid I-A-region gene products function effectively in antigen presentation. J Exp Med 1980, 152: 759PubMedCrossRefGoogle Scholar
  12. 12).
    Fisher A, et al: An influenza virus matrix proteinspecific human T cell line with helper activity for in vitro anti-hemaggulutinin antibody production. Eur J Immunol 1982, 12: 844CrossRefGoogle Scholar
  13. 13).
    Lamb JR, et al: In vitro influenza virus-specific and HLA-restricted induction of helper activity mediated by cloned human T lymphocytes. J Immunol 1982, 129: 1465PubMedGoogle Scholar
  14. 14).
    Celis E, et al: Hepatitis B virus-reactive human T lymphocyte clones: antigen specificity and helper function for antibody synthesis. J Immunol 1984, 132: 1511PubMedGoogle Scholar
  15. 15).
    Nonomura A, et al: Activities of lymphocytes mediating antibody-dependent cell-mediated cytotoxicity in patients with chronic active hepatitis. Tohoku J Exp Med 1981, 135: 63PubMedCrossRefGoogle Scholar
  16. 16).
    Nonomura A, et al: T cell-mediated cytotoxicity against HBsAg coated Chang cells in patients with chronic hepatitis: evidence for cytotoxicity mediated by delayed hypersensitivity T cell reaction. Tohoku J Exp Med 1982, 138: 139PubMedGoogle Scholar
  17. 17).
    Chang TW, et al: In vitro response to HBsAg of peripheral blood lymphocytes from recipients of hepatitis B vaccine. Hepatology 1984, 4: 824PubMedGoogle Scholar
  18. 18).
    Roberts IM, et al: T-cell dependence of immune response to hepatitis B antigen in mice. Nature 1975, 254: 606PubMedCrossRefGoogle Scholar
  19. 19).
    Sredni B, et al: Human T cell clones reactive with soluble antigens: methodology, specificity, and MHC restriction, in “Isolation, Characterization, and Utilization of T Lymphocyte Clones”, by Fathman CG and Fitch FW. Academic Press, New York, 1982; 439Google Scholar
  20. 20).
    Kimoto M, et al: Cloning of soluble antigen reactive murine T inducer (Th) cells, in “Isolation, Characterization, and Utilization of T Lymphocyte Clones”, by Fathman CG and Fitch FW. Academic Press, New York, 1982; 527Google Scholar
  21. 21).
    Celis E, et al: Antibodies to hepatitis B surface antigen potentiate the response of human T lymphocyte clones to the same antigen. Science 1984, 224: 297PubMedCrossRefGoogle Scholar
  22. 22).
    Muraguchi A, et al: Differential sensitivity of human B cell subsets to activation signals derived by anti-μ antibody and proliferative signals delivered by a monoclonal B cell growth factor. J Exp Med 1983, 157: 530PubMedCrossRefGoogle Scholar
  23. 23).
    Boyd AW, et al: Structural and functional characterization of IL 2 receptors on activated human B cells. J Immunol 1985, 134: 2387PubMedGoogle Scholar
  24. 24).
    Mittler R, et al: Activated human B cells display a functional IL 2 receptor. J Immunol 1985, 134: 2393PubMedGoogle Scholar

Copyright information

© The Japanese Society of Gastroenterology 1987

Authors and Affiliations

  • Akitaka Nonomura
    • 1
  • Goroku Ohta
    • 1
  1. 1.Department of Pathology, School of MedicineKanazawa UniversityIshikawaJapan

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