Gastroenterologia Japonica

, Volume 14, Issue 5, pp 417–424 | Cite as

Conversion of 7-ketolithocholic acid to ursodeoxycholic acid by human intestinal anaerobic microorganisms: Interchangeability of chenodeoxycholic acid and ursodeoxycholic acid

  • Shushi Higashi
  • Toshiaki Setoguchi
  • Taketo Katsuki
Original Article


Chenodeoxycholic acid, ursodeoxycholic acid and 7-ketolithocholic acid were incubated with human intestinal bacteria (source: 4 healthy males) at 37‡C for 72 hours in an anaerobic condition. The bile acids of the products in culture medium were identified by three independent methods, thin layer chromatography, gas-liquid chromatography and GLC-mass spectrometry.

Lithocholic acid, ursodeoxycholic acid and 7-ketolithocholic acid were observed in the culture of chenodeoxycholic acid. Lithocholic acid, chenodeoxycholic acid and 7-ketolithocholic acid were observed in the culture of ursodeoxycholic acid. Chenodeoxycholic acid and ursodeoxycholic acid were produced from 7-ketolithocholic acid. These data may suggest that chenodeoxycholic acid and ursodeoxycholic acid are interconvertible via 7-ketolithocholic acid by the mixed culture of human intestinal microorganisms under an anaerobic condition.

Key Words

bile acid oxidoreduction intestinal microorganisms anaerobic culture 


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Copyright information

© The Japanese Society of Gastroenterology 1979

Authors and Affiliations

  • Shushi Higashi
    • 1
  • Toshiaki Setoguchi
    • 1
  • Taketo Katsuki
    • 1
  1. 1.The First Department of SurgeryMiyazaki Medical CollegeMiyazakiJapan

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