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Studies on circulating soluble immune complexes of the liver disease 1. Inhibition assay of polyclonal rheumatoid factor binding to IgG-sepharose

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Summary

Polyclonal rheumatoid factor (pRF) was isolated from sera seropositive for RF and it was radioiodinated with lactoperoxidase method. Using the125I-pRF and IgG-p-azobenzamidoethyl Sepharose 6B, a competitive inhibition radioassay for detecting immune complexes is described. The assay can be performed in 90 min utilizing 10ΜI of serum with good reproducibility and endogenous RF in test serum did not interfere the reaction. The test can detect complexes nearly to 8s and as low as 0.1Μg/ml of aggregated human IgG. Decomplementation by heating test sera is unnecessary. The assay was performed at 4‡C but less inhibition activity was obtained when it was carried out at 24‡C.

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References

  1. Agnello V, Winchester RJ, Kunkel HG: Precipitin reactions of the Clq component of complement with aggregated gamma globulin and immune complexes in gel difusion. Immunol 19: 909–919, 1970

    CAS  Google Scholar 

  2. Svehag SE: A solid-phase radioimmunoassay for Clqbinding immune complexes: 1. IgG as indicatormolecule. Scand J Immunol 4: 687–697, 1975

    Article  PubMed  CAS  Google Scholar 

  3. Sobel AT, Bokisch VA, Muller-Eberhrd HJ: Clq deviation test for the detection of immune complexes, aggregates of IgG, and bacterial products in human serum. J Exp Med l45: 139–150, 1975

    Article  Google Scholar 

  4. Farrel H, Sogaard H, Svehag SE: Detection of IgG aggregates or immune complexes using solid-phase Clq and protein A-rich Staphylococcus aureus as an indicator system. Scand J Immunol 4: 673–680, 1975

    Article  Google Scholar 

  5. Zubler RH, Nydegger V, Perrin LH, et al: Ciculating and intraarticular immune complexes in patients with rheumatoid arthritis: correlation of125I-Clq binding activity with monoclonal and biological features of the disease. J Clin Invest 57: 1308–1319, 1976

    PubMed  CAS  Google Scholar 

  6. Ahlstedt S, Hanson LA, Wadsworth C: A Clq immunosorbent assay compared with thin-Iyer gel diffusion for measuring IgG aggregates. Scand J Immunol 5:293–298, 1976

    Article  PubMed  CAS  Google Scholar 

  7. Hay FC, Nineham LJ, Roitt IM: Routine assay for the detection of immune complexes of known immunoglobulin class using solid phase Clq. Clin Exp Immunol 24:296–400, 1976

    Google Scholar 

  8. Luhrhum AZ, Cambiano CL, Masson PL, et al: Detection of circulating antigen-antibody complexes by their inhibitory effect on the agglutination of IgGcoated particles by rheumatoid factor or Clq. Clin Exp Immunol 25: 212–226, 1976

    Google Scholar 

  9. Teshima H, Wanebo H, Pinsky C, et al: Circulating immune complexes detected by125I-Clq deviation test in sera of cancer patients. J Clin Invest 59: 1134–1142, 1977

    PubMed  CAS  Google Scholar 

  10. Lieberman P, Rice MC, Mallette JE Jr: Studies of urticaria and acute serum sickness with the Clq precipitin test. Arch Intern Med 137: 440–442, 1977

    Article  PubMed  CAS  Google Scholar 

  11. Fletcher Ds, Tsau-Yen Lin: Quantitation of immune complexes by competitive inhibition of binding of Clq to insoluble IgG aggregates. J Immunol Methods 15: 39–45, 1977

    Article  PubMed  CAS  Google Scholar 

  12. Gabriel A, Agnello V: Detection of immune complexes: the use of radioimmunoassay with Clq and monoclonal rheumatoid factor. J Clin Invest 59: 990–1001, 1977

    Article  PubMed  CAS  Google Scholar 

  13. Theofilopoulos AN, Wilson CB, Bokisch VA, et al: Binding of soluble immune complexes to human lymphoblastoid cells. II. Use of Raji cells to detect circulating immune complexes in animal and human sera. J Exp Med 140: 1233–1244, 1974

    Google Scholar 

  14. Onyewotu II, Holborrow, Johnson GD: Detection and radioassay of soluble circulating immune complexes using guinea pig peritonal exudate cells. Nature 248: 156–159, 1974

    Article  PubMed  CAS  Google Scholar 

  15. Cowdery JS Jr, Treadwell PE, Fritz RB: A radioimmunoassay for human antigen-antibody complexes in clinical materials. J Immunol 114: 5–9, 1975

    PubMed  CAS  Google Scholar 

  16. Luthra HS, McDuffie FC, Hunder GG, et al: Immune complexes in sera and synovial fluids of patients with rheumatoid arthritis: radioimmunoassay with monoclonal rheumatoid factor. J Clin Invest 56: 458–466, 1975

    Article  PubMed  CAS  Google Scholar 

  17. Nydegger UE, Lambert PH, Gerber H, et al: Circulating immune complexes in the serum in systemic lupus erythematosus and carriers of hepatitis B antigen: quantitation by binding to radiolabelled Clq. J Clin Invest 54: 297–309, 1974

    PubMed  CAS  Google Scholar 

  18. Rossen RD, Reisberg MA, Hersh EM, et al: The Clq binding test for soluble immune complexes: clinical correlations obtained in patients with cancer. J Natl Cancer Inst 58: 1205–1215, 1977

    PubMed  CAS  Google Scholar 

  19. Lurhuma AZ, Riccomi H, Masson PL: The occurence of circulating immune complexes and viral antigens in idiopathic thrombocytopenic purpura. Clin Exp Immunol 28: 49–55, 1977

    PubMed  CAS  Google Scholar 

  20. Thomas HC, Villiers DD, Potter B, et al: Immune complexes in acute and chronic liver disease. Clin Exp Immunol 31: 150–157, 1978

    PubMed  CAS  Google Scholar 

  21. Winchester RJ, Kunkel HG, Agnello V: Occuerence of gamma-globulin complexes in serum and joint fluids of rheumatoid arthritis patients: use of monoclonal rheumatoid factors as reagents for their demonstration. J Exp Med 134: 286s-295s, 1971

    PubMed  CAS  Google Scholar 

  22. Maoke JL, Kageller WV, Cimo PL, et al: Intravascular hemolysis, thrombocytopenia, leukopenia and circulating immune complexes after jejunal-ileal bypass surgery. Ann Intern Med 86: 576–568, 1972

    Google Scholar 

  23. Wands JR, Dienstag JL, Bhan AK, et al: Circulating immune complex and complement activation in primary biliary cirrhosis. N Eng J Med 298: 233–237, 1978

    Article  CAS  Google Scholar 

  24. Bing DH: Purification of the first component and its subunit Cl esterase. Methods Enzymol 34: 731–746, 1974

    PubMed  CAS  Google Scholar 

  25. Cuatrecasas P, Antinsen CB: Affinity chromatography. Methods Enzymol 34: 731–746, 1974

    Google Scholar 

  26. Mancini G, Carbonara AO, Hermans JF: Immunochemical quantitation of antigens by single radial immunodifusion. Immunochemistry 2: 235–254, 1965

    Article  PubMed  CAS  Google Scholar 

  27. Lowry OH, Rosenbrough NJ, Farr AL, et al: Protein measurement with the Folin phenol reagent. J Biol Chem l93:265–267, 1951

    Google Scholar 

  28. Heusser C, Boesman M, Nordin JH, et al: Effect of chemical and enzymatic radioiodination on in vitro human Clq activities. J Immunol 110: 820–828, 1973

    PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. The First Department of Internal Medicine, Okayama University Medical School, 2-5-1 Shikata-cho, 700, Okayama, Japan

    Jin Narumoto M.D., Terukatsu Arima M.D. & D.Sci., Kensuke Kunishi M.D., Takashi Yasuhara M.D., Kinichiro Suwaki M.D., Hiroyuki Shimomura M.D. & Hideo Nagashima M.D.

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  1. Jin Narumoto M.D.
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  2. Terukatsu Arima M.D. & D.Sci.
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  3. Kensuke Kunishi M.D.
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  4. Takashi Yasuhara M.D.
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  5. Kinichiro Suwaki M.D.
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  6. Hiroyuki Shimomura M.D.
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  7. Hideo Nagashima M.D.
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Narumoto, J., Arima, T., Kunishi, K. et al. Studies on circulating soluble immune complexes of the liver disease 1. Inhibition assay of polyclonal rheumatoid factor binding to IgG-sepharose. Gastroenterol Jpn 14, 611–616 (1979). https://doi.org/10.1007/BF02773720

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  • DOI: https://doi.org/10.1007/BF02773720

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