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Plant Molecular Biology Reporter

, Volume 22, Issue 1, pp 93–93 | Cite as

Total RNA isolation fromPicea mariana dry seed

  • Helen H. Tai
  • Céline Pelletier
  • Tannis Beardmore
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Abstract

Isolating RNA from dry conifer seeds can be difficult because of a number of interfering compounds present in seeds. We describe a protocol for total RNA isolation from black spruce dry seeds, which is an adaptation of a method used for mouse myeloma tissues. The extraction relies on selective precipitation of RNA by using lithium chloride.

Key words

conifer lithium chloride Picea RNA isolation seed spruce 

Copyright information

© Springer 2004

Authors and Affiliations

  • Helen H. Tai
    • 1
  • Céline Pelletier
    • 1
  • Tannis Beardmore
    • 1
  1. 1.Canadian Forest ServiceNatural Resources CanadaFrederictonCanada

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