A protocol for high-throughput extraction of DNA from rice leaves
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Current DNA isolation methods have limitations between speed and purity in high-throughput molecular genetic analysis such as gene mapping and marker-assisted selection programs. We have optimized a simple and rapid method for isolating high-quality genomic DNA from rice that significantly minimizes time and the use of laboratory materials. One person can process as many as 384 samples in 2 h. The isolated DNA is suitable for polymerase chain reaction-based techniques and is stable for no less than 6 mo of storage at 4°C.
Key wordsDNA isolation map-based cloning PCR amplification rice
high-efficiency genome scanning
polymerase chain reaction
single nucleotide polymorphism
simple sequence repeats
sequence tagged site
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