Plant Molecular Biology Reporter

, Volume 23, Issue 3, pp 291–295 | Cite as

A protocol for high-throughput extraction of DNA from rice leaves

  • Xin Xu
  • Shinji Kawasaki
  • Tatsuhito Fujimura
  • Chuntai Wang


Current DNA isolation methods have limitations between speed and purity in high-throughput molecular genetic analysis such as gene mapping and marker-assisted selection programs. We have optimized a simple and rapid method for isolating high-quality genomic DNA from rice that significantly minimizes time and the use of laboratory materials. One person can process as many as 384 samples in 2 h. The isolated DNA is suitable for polymerase chain reaction-based techniques and is stable for no less than 6 mo of storage at 4°C.

Key words

DNA isolation map-based cloning PCR amplification rice 



hexadecyltrimethylammonium bromide


high-efficiency genome scanning


polymerase chain reaction


single nucleotide polymorphism


simple sequence repeats


sequence tagged site


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Copyright information

© Springer 2005

Authors and Affiliations

  • Xin Xu
    • 1
    • 3
  • Shinji Kawasaki
    • 2
  • Tatsuhito Fujimura
    • 3
  • Chuntai Wang
    • 1
    • 2
  1. 1.Key Laboratory for Biotechnology of National Commission for Nationalities, College of Life ScienceSouth-Central University for NationalitiesWuhanP.R., China
  2. 2.Plant Physiology DepartmentNational Institute of Agrobiological SciencesTsukuba, IbarakiJapan
  3. 3.Laboratory of Plant Genetic EngineeringUniversity of TsukubaTsukuba, IbarakiJapan

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