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Plant Molecular Biology Reporter

, Volume 22, Issue 2, pp 189–194 | Cite as

A rapid method for the characterisation of transgene junctions

  • Derek Matthews
Protocols

Abstract

Presented here is a simple method that enables amplification of the DNA immediately surrounding the junction between an inserted tDNA and the host genome, without prior sequence information.

Key words

approved event detection GMO junction PCR tDNA 

Abbreviations

EDTA

ethylenediaminetetraacetic acid

GM

genetically modified

Taq

Thermus aquaticus DNA polymerase

tDNA

transfer DNA

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References

  1. Theuns I, Windels P, De Buck S, Depicker A, van Bockstaele E, De Loose M (2002) Identification and characterization of T-DNA inserts by T-DNA fingerprinting. Euphytica 123: 75–84.CrossRefGoogle Scholar

Copyright information

© International Society for Plant Molecular Biology 2004

Authors and Affiliations

  1. 1.Research Strategy and Support UnitNIABCambridgeUK

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