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Plant Molecular Biology Reporter

, Volume 23, Issue 1, pp 39–51 | Cite as

Evaluation of genetic diversity and germ plasm identification of 44 species, clones, and cultivars from 5 sections of the genusPopulus based on amplified fragment length polymorphism analysis

  • Chun Jiang Zhou
  • Hong Zhu Song
  • Jin Hua Li
  • Jian Wei Sun
  • Min De Jin
  • Qi Wen Zhang
  • Bin Wang
Genetic Resources

Abstract

The genusPopulus L. (Salicaceae) can be divided into 5 sections with distribution throughout the world. Accurate identification ofPopulus clones and species is essential for effective selection, breeding, and management of genetic resources. In this study, amplified fragment length polymorphism (AFLP) analysis, which was reported as a reliable technique with high efficiency in detecting polymorphism, was used to conduct analyses of genetic diversity and variety identification of 44 species, clones, and cultivars ofPopulus that represent a wide range of breeding and commercially available germplasms. Cluster analysis of the 44 samples was carried out, and a dendrogram of genetic relatedness was developed on the basis of the AFLP data. DNA fingerprints of the 44 samples were developed from 12 selected bands amplified with 2 primer combinations (M-CAG/E-TA and M-CAG/E-TC). Each sample has its unique fingerprint pattern and can be distinguished from the others. Furthermore, 1 specific AFLP band of the cultivarPopulus canadensis cl. Guariento coming from fragments amplified by primer combination M-CTC/E-AG was successfully converted into a sequence-characterized amplified region (SCAR) marker. The results indicate that AFLP analysis should be considered as the preferred technique for the study of polymorphism inPopulus. This research is the first report concerning the use of AFLP analysis in genetic diversity and germplasm identification among all sections ofPopulus.

Key words

amplified fragment length polymorphism cluster analysis genetic diversity germplasm identification Populus sequence characterized amplified region 

Abbreviations

AFLP

amplified fragment length polymorphism

CTAB

cetyltrimethylammonium bromide

EDTA

ethylene diamine tetraacetic acid

MAS

marker-assisted selection

PAGE

polyacrylamide gel electrophoresis

RAPD

random-amplified polymorphic DNA

RFLP

restriction fragment length polymorphism

SCAR

sequence-characterized amplified region

SSR

simple sequence repeat

UPGMA

unweighted pair group method using arithmetic means

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References

  1. Barrett BA Kidwell KK (1998) AFLP-based genetic diversity assessment among wheat cultivars from the pacific northwest. Crop Sci 38: 1261–1271.CrossRefGoogle Scholar
  2. Bradshaw HD and Stettler RF (1995) Molecular genetics of growth and development inPopulus. IV. Mapping QTLs with large effects on growth, form, and phenology traits in a forest tree. Genetics 139: 963–973.PubMedGoogle Scholar
  3. Brunner AM, Busov VB, and Strauss SH (2004) Poplar genome sequence: functional genomics in an ecologically dominant plant species. Trends Plant Sci 9: 49–56.PubMedCrossRefGoogle Scholar
  4. Che KP, Liang CY, Wang YG, Jin DM, Wang B, Xu Y, Kang GB, and Zhang HY (2003) Genetic assessment of watermelon germplasm using the AFLP technique. HortScience 38: 81–84.Google Scholar
  5. Chen YH, Jia JH, Li CY, Jin DM, Weng ML, and Wang B (2000) Rice seed identification by computerized AFLP-DNA fingerprinting analysis. J Agric Biotechnol 8: 222–224.Google Scholar
  6. Dayanandan S, Rajora OP, and Bawa KS (1998) Isolation and characterization of microsatellites in trembling aspen (Populus tremuloides). Theor Appl Genet 96: 950–956.CrossRefGoogle Scholar
  7. Eckenwalder JE (1996) Systematics and evolution ofPopulus In: Stettler RF, Bradshaw HD, Heilman PE, and Hinckley TM (eds), Biology ofPopulus and its Implications for Management and Conservation, pp 7–32, NRC Research, Ottawa.Google Scholar
  8. Franco J, Crossa J, Ribaut JM, Bertran J, Warburton ML, and Khairallah M (2001) A method for combining molecular markers and phenotypic attributes for classifying plant genotypes. Theor Appl Genet 103: 944–952.CrossRefGoogle Scholar
  9. Gu RS, Fonseca S, Puskás LG, Hackler LJr, Zvara A, Dudits D, and Pais MS (2004) Transcript identification and profiling during salt stress and recovery ofPopulus euphratica. Tree Physiol 24: 265–276.PubMedCrossRefGoogle Scholar
  10. Hill M, Witsenboer H, Zabeau M, Vos P, Kesseli R, and Michelmore R (1996) PCR-based fingerprinting using AFLPs as a tool for studying genetic relationships in Lactuca spp. Theor Appl Genet 93: 1202–1210.CrossRefGoogle Scholar
  11. Hong T, Ma ZL, and Chen JS (1987) Floral morphology ofPopulus lasiocarpa Oliv. and its phylogenetic position inPopulus. Acta Botanic Sinica 29: 236–241.Google Scholar
  12. Li KY, Huang MR, and Wang MX,(1997) Study on origin ofPopulus tomentosa Carr. Acta Phytotax Sin 35(1): 24–31.Google Scholar
  13. Li KY, Huang MR, Wang MX, Chen DM, and He ZX (1996) Study on DNA polymorphisms and phylogenetics ofPopulus: Aigeiros, Tacamahaca and Leuce section. J Nanjing Forestry University 20(1): 6–11.Google Scholar
  14. Liu Z and Furnier GR (1993) Inheritance and linkage of allozymes and restriction fragment length polymorphisms in trembling aspen. J Hered 84: 419–424.Google Scholar
  15. Murry HG and Thompson WF (1980) Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res 8: 4321–4325.CrossRefGoogle Scholar
  16. Nei M and Li WH (1979) Mathematical model for studying genetic variation in trems of restriction endonuclease. Proc Natl Acad Sci 76: 5269–5273.PubMedCrossRefGoogle Scholar
  17. Park YH, West MA, and Clair DA (2004) Evaluation of AFLPs for germplasm fingerprinting and assessment of genetic diversity in cultivars of tomato (Lycopersicon esculentum L.). Genome 47: 510–518.PubMedCrossRefGoogle Scholar
  18. Rahman MH and Rajora OP (2002) Microsatellite DNA fingerprinting, differentiation, and genetic relationships of clones, cultivars, and varieties of six poplar species from three sections of the genusPopulus. Genome 45: 1083–1094.PubMedCrossRefGoogle Scholar
  19. Rajora OP (1989) Genetic structure and identification ofPopulus deltoides Marsh. clones based on allozymes. Genome 32: 440–448.CrossRefGoogle Scholar
  20. Rajora OP and Rahman MH (2003) Microsatellite DNA and RAPD fingerprinting, identification and genetic relationship of hybrid poplar (Populus × canadensis) cultivars. Theor Appl Genet 106: 470–477.PubMedGoogle Scholar
  21. Sigurdsson V, Anamthawat JK, and Sigurgeirsson A (1995) DNA fingerprinting ofPopulus trichocarpa clones using RAPD markers. New Forests 10: 197–206.CrossRefGoogle Scholar
  22. Taylor G (2002)Populus: arabidopsis for forestry. Do we need a model tree? Ann Bot 90: 681–689.PubMedCrossRefGoogle Scholar
  23. Vos P, Hogers R, Bleeker M, Reijans M, van de Lee T, Hornes M, Frijters A, Pot J, Peleman J, Kuiper M, and Zabeau M (1995) AFLP: a new technique for DNA finger-printing. Nucleic Acids Res 123: 4407–4414.CrossRefGoogle Scholar
  24. Wang B, Li CY, Zhang HG, Fu JM, Yang RC, and Nguyen HT (1999a) Primary study of rice AFLP analysis-optimization of reaction condition and analysis of thermosensitive genic male sterile rice allelic mutant lines. Acta Botanic Sinic 41: 502–507.Google Scholar
  25. Wang B, Zhang CL, Weng ML, Gao HY, Jin DM, Sun ZL, and Sun SM (1999b) Conversion of RAPD markers into SCAR markers of maize inbred line. High Tech Letters 9(3): 45–47.Google Scholar
  26. Xu SB, Tao YF, Yang ZQ, and Chu JY (2002) A simple and rapid method used for silver staining and gel preservation. Hereditas (Beijing) 24: 338–336.Google Scholar
  27. Yin TM, Huang MR, Wang MX, Zhu LH, Zeng ZB, and Wu RL (2001) Preliminary interspecific genetic maps of thePopulus genome constructed from RAPD markers. Genome 44: 602–609.PubMedGoogle Scholar

Copyright information

© International Society for Plant Molecular Biology 2005

Authors and Affiliations

  • Chun Jiang Zhou
    • 1
    • 2
  • Hong Zhu Song
    • 3
  • Jin Hua Li
    • 3
  • Jian Wei Sun
    • 1
  • Min De Jin
    • 1
  • Qi Wen Zhang
    • 3
  • Bin Wang
    • 1
  1. 1.State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijingChina
  2. 2.Graduate School of the Chinese Academy of SciencesBeijingChina
  3. 3.Research Institute of ForestryChinese Academy of ForestryBeijingChina

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