Advertisement

Plant Molecular Biology Reporter

, Volume 21, Issue 4, pp 467–467 | Cite as

DNA isolation from fresh, dry plant samples with highly acidic tissue extracts

  • Dnyaneshwar Warude
  • Preeti Chavan
  • Kalpana Joshi
  • Bhushan Patwardhan
Publish by Abstract

Abstract

Current DNA isolation methods are limited in their ability to obtain quality and/or quantity DNA from plants, such asEmblica officinalis, Terminalia belerica, andTerminalia chebula, which have low pH and high amounts of secondary metabolites in tissue extracts. Our modified DNA isolation method yields good-quality, high-molecular-weight DNA that is free of contaminants and colored pigments and is suitable for PCR amplification. This method is also useful for isolating DNA from dry powders.

Key words

DNA isolation Emblica officinalis low pH PCR Terminalia belerica Terminalia chebula triphala churna 

Copyright information

© International Society for Plant Molecular Biology 2003

Authors and Affiliations

  • Dnyaneshwar Warude
    • 1
  • Preeti Chavan
    • 1
  • Kalpana Joshi
    • 1
  • Bhushan Patwardhan
    • 1
  1. 1.Interdisciplinary School of Health SciencesUniversity of PunePuneIndia

Personalised recommendations