Isolation of Plasmid DNA rescued from single colonies ofAgrobacterium tumefaciens by means of rolling circle amplification
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We report a simple method to isolate plasmids from single colonies ofAgrobacterium tumefaciens by means of rolling circle amplification. The amplified DNA can be digested by restriction enzymes for plasmid verification and transformed intoEscherichia coli for plasmid rescue. Compared with conventional procedures, this method eliminates liquid culturing ofAgrobacterium cells and subsequent DNA isolation and enables large-scale plasmid analyses.
Key wordsAgrobacterium tumefaciens Escherichia coli plasmid DNA rolling circle amplification
rolling circle amplification
tandem affinity purification
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