Plant Molecular Biology Reporter

, Volume 21, Issue 4, pp 411–415 | Cite as

Isolation of Plasmid DNA rescued from single colonies ofAgrobacterium tumefaciens by means of rolling circle amplification

  • Xiuhua Chen
  • Xiaodong Ding
  • Wen-Yuan Song


We report a simple method to isolate plasmids from single colonies ofAgrobacterium tumefaciens by means of rolling circle amplification. The amplified DNA can be digested by restriction enzymes for plasmid verification and transformed intoEscherichia coli for plasmid rescue. Compared with conventional procedures, this method eliminates liquid culturing ofAgrobacterium cells and subsequent DNA isolation and enables large-scale plasmid analyses.

Key words

Agrobacterium tumefaciens Escherichia coli plasmid DNA rolling circle amplification 





rolling circle amplification


tandem affinity purification


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Copyright information

© International Society for Plant Molecular Biology 2003

Authors and Affiliations

  • Xiuhua Chen
    • 1
  • Xiaodong Ding
    • 1
  • Wen-Yuan Song
    • 1
  1. 1.Department of Plant Pathology, Plant Molecular and Cellular Biology ProgramUniversity of FloridaGainesvilleUSA

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