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Plant Molecular Biology Reporter

, Volume 19, Issue 2, pp 183–183 | Cite as

A rapid and high yielding DNA miniprep for cotton (Gossypium spp.)

  • Haiwen Li
  • Jinhua Luo
  • John K. Hemphill
  • Jau-Tay Wang
  • Jean H. Gould
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Abstract

A rapid DNA minipreparation method was developed for cotton and yields 500–600 μg DNA from 1.0 g fresh leaf tissue. Cotton DNA extracted using this method is completely digested with restriction enzymes, supports PCR and Southern DNA analyses and was used successfully in these applications.

Key words

DNA extraction Gossypium minipreparation 

Copyright information

© International Society for Plant Molecular Biology 2001

Authors and Affiliations

  • Haiwen Li
    • 1
  • Jinhua Luo
    • 2
  • John K. Hemphill
    • 1
  • Jau-Tay Wang
    • 3
  • Jean H. Gould
    • 1
  1. 1.Department of Forest ScienceTexas A&M UniversityCollege StationUSA
  2. 2.AgriVitis, Inc.San Juan BautistaUSA
  3. 3.TaichungTaiwan

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