Malic enzyme: its purification and characterization fromMucor circinelloides and occurrence in other oleaginous fungi

  • J. Savitha
  • J. P. Wynn
  • C. Ratledge


Malic enzyme was purified 43-fold from Mucor circinelloides. The enzyme was dependent on Mg2+ or Mn2+ for activity, was not active with Dmalate and had a pH optimum at 7.8. The apparent Km values for malate and NADP+ were 488 ΜM and 41 Μm respectively. The Mr of the native enzyme was 160 kDa. Five metabolic analogues of malate: oxaloacetate, tartronic acid, 1-methylenecyclopropane trans-2,3-dicarboxyIic acid, malonic acid and glutaric acid, were found to inhibit malic enzyme activity at 10 mM. Four oleaginous fungi, Mucor circinelloides, Mortierella alpina, Mortierella elongata and Pythium ultimum, were also examined, all possessed a soluble malic enzyme, two also possessed a microsomal malic enzyme.

Key words

Fungi malic enzyme Mucor oleaginicity 


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Copyright information

© Rapid Science Publishers 1997

Authors and Affiliations

  • J. Savitha
    • 2
  • J. P. Wynn
    • 1
  • C. Ratledge
    • 1
  1. 1.Department of Applied BiologyUniversity of HullUK
  2. 2.Department of MicrobiologyBangalore UniversityBangaloreIndia

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