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Bulletin of Experimental Biology and Medicine

, Volume 123, Issue 2, pp 187–190 | Cite as

Cytological analysis of the histone component of hepatocyte nuclear chromatin in rats with impaired vagus innervation of the liver

  • I. V. Shcherbo
  • T. K. Dubovaya
  • A. V. Grigor’eva
  • O. V. Bul’chuk
  • G. V. Cherkesova
Experimental Biology
  • 19 Downloads

Abstract

The supramolecular arrangement of rat hepatocyte nuclear chromatin is studied by differentiated staining of the histone component after bilateral subdiaphragmal vagotomy in intact and phenobarbital-induced animals. The spectrum of hepatocyte nuclei staining is not altered in denervated liver; however, pronounced shifts in the quantitative ratio of different types of staining occur 1–2 weeks after denervation. One month after the operation, the hepatocyte population is almost the same as in the control regarding the staining pattern and quantitative ratio of its variants. In the phenobarbital group, the spectrum of hepatocyte staining does not change compared with intact control and vagotomized animals at all terms after surgery. This may be due to the restructuring of the nucleohistone—chromatin complex or lack of such restructuring.

Key Words

chromatin histones hepatocyte vagotomy 

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Copyright information

© Plenum Publishing Corporation 1997

Authors and Affiliations

  • I. V. Shcherbo
    • 1
  • T. K. Dubovaya
    • 1
  • A. V. Grigor’eva
    • 1
  • O. V. Bul’chuk
    • 1
  • G. V. Cherkesova
    • 1
  1. 1.Russian State Medical UniversityMoscow

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